Fig 1.
Radiographic appearance of the examined ossification centres.
A. Saarloos Wolfdog, F, 6 weeks; medio-lateral view of the right forelimb; B. Saarloos Wolfdog, F, 8 weeks; medio-lateral view of the right hindlimb. Green scale bar: Distance between two hyphens is of 1 cm. Abbreviations: Sca = supraglenoid tubercule; HumP = proximal epiphysis of the humerus; HumE = epiphysis of medial epicondyle of the humerus; HumD = distal epiphysis of the humerus; RadP = proximal epiphysis of the radius; RadD = distal epiphysis of the radius; UlnO = olecranon tuber; UlnD = distal epiphysis of the ulna; Car = accessory carpal bone; Fem = distal epiphysis of the femur; Pat = patella; Fab = Fabellae; Pop = Popliteal bones; Fib = proximal epiphysis of the fibula; TibP = condyles of the proximal epiphysis of the tibia; TibT = tibial tuberosity; TibD = distal epiphysis of the tibia; Tar = calcaneal tuber.
Fig 2.
A. White Swiss Shepherd Dog, F, 10 weeks, medio-lateral view of the right forelimb; B. Saarloos Wolfdog, F, 16 weeks, medio-lateral view of the right hindlimb. Diaphyseal length measurements (white lines) were performed by drawing a perpendicular line to the distal diaphyseal end of the bone and measuring the ossified diaphysis at its maximum length. The OC areas (outlined by the different coloured lines) were measured by drawing freehand the outline of the corresponding radiopaque area. Green scale bar: Distance between two hyphen is of 1 cm. Abbreviations: aSca = area of the supraglenoid tubercule; aHumP = area of the proximal epiphysis of the humerus; aUlnO = area of the olecranon tuber; aRadD = area of the distal epiphysis of the radius; lHum = diaphyseal length of the humerus; lRad = diaphyseal length of the radius; lUln = diaphyseal length of the ulna; lTib = diaphyseal length of the tibia; aPat = area of the patella; aFab = area of the fabellae; aPop = area of the popliteal bones; aFib = area of the proximal epiphysis of the fibula; aTibT = area of the tibial tuberosity; aTar = area of the calcaneal tuber.
Fig 3.
Representative series of radiographic images of the forelimb skeletal development.
Saarloos Wolfdog, medio-lateral view of the right forelimb. A) 6 weeks of age; B) 8 weeks of age; C) 10 weeks of age; D) 12 weeks of age; E) 14 weeks of age; F) 16 weeks of age. Green scale bar: Distance between two hyphen is of 1 cm.
Fig 4.
Representative series of radiographic images of the hindlimb skeletal development.
Saarloos Wolfdog, medio-lateral view of the right hindlimb. A) 6 weeks of age; B) 8 weeks of age; C) 10 weeks of age; D) 12 weeks of age; E) 14 weeks of age; F) 16 weeks of age. Green scale bar: Distance between two hyphen is of 1 cm.
Table 1.
Breed OC score at each timepoint calculated by summing the number of OCs present out of 14 (HumP, HumD, HumE, RadP, RadD, UlnO, UlnD, Car, Fem, Pat, TibP, TibT, TibD, Tar).
Table 2.
Spearman’s rank correlation between the relative increase in the radiographic measurements and age and body weight.