Fig 1.
Gross lesions of CLA in dromedary camel.
(A) superficial abscesses on the right prefemoral lymph nodes (iliaci externi) (arrow). (B) Incised right inferior cervical lymph node (cervicales laterals) showing multiple caseated caseous pyogranulomas containing yellowish-cheesy martials. (C) Liver showing multifocal-diffused pyogranulomas contained yellowish- cheesy pus. (D) Thoracic cavity showed multiple open large abscesses in the lung with adhesion to the chest wall (arrow).
Fig 2.
Histologic section of liver and lymph node of dromedary camel infected with CLA.
(A) Liver section showing central caseous necrotic core admixed bacterial colonies and infiltration of polymorphonuclear cells, lymphocytes, plasma cells and macrophages (3), surrounded by pyogenic membrane (2), and fibrous capsule (1), H&E stain, 20×. (B) Lymph node section showing central caseous necrotic abscess (3), surrounded by inflammatory cells (2) and bound by a thick fibrous capsule (1), H&E stain 20 ×.
Fig 3.
Gel image of PCR amplified 16S rRNA gene (partial, ~ 900 bp) for the 12 samples shown in Table 1.
Fig 4.
Images of mapping of sequence reads for 4 representative samples (sample 1, 7, 9 and 12) to the reference genome of Corynebacterium pseudotuberculosis (Accession number: NZ_CP054555.1). The sequence reads mapped to reference genome between positions 619301–620091 (791 bp), 619232–620012 (781 bp), 619233–620023 (791 bp) and 619293–620093 (801 bp) for A (sample 1), B (sample 7), C (sample 9) and D (sample 12) respectively.
Table 1.
The top three close sequences of the Corynebacterium pseudotuberculosis to our isolates, retrieved from BLAST analysis.
Table 2.
GenBank accession numbers for the 16S rRNA gene partial sequence of Corynebacterium pseudotuberculosis isolated from dromedary camels in UAE (2015–2020) along with the length of each isolate sequence and the span on the reference genome Corynebacterium pseudotuberculosis (Accession number: NZ_CP054555.1) during mapping.
Fig 5.
Phylogenetic tree by Maximum Likelihood method.
The tree based on the sequence of 16S rRNA gene was constructed by MEGAX software [38] using Maximum Likelihood method based on the Kimura 2-parameter model [39]. We used a Boostrap value of 1000 repetition, Corynebacterium pseudotuberculosis isolates (Black Square bordered) were included in this study as well as GenBank reference samples of the subspecies ovis and equi, a reference sequence of the Corynebacterium ulcerans (Blue), Corynebacterium renale (Red) and Corynebacterium diphtheria (Pink).