Fig 1.
Species differentiation based on the Tm values.
Melting curves of amplicons post real-time PCR from P. falciparum (red), P. malariae (green), P. ovale (lilac) and P. vivax (grey).
Table 1.
Performance of the reference samples in the standard nested PCR and in the novel FRET-qPCR.
Fig 2.
Boxplots of the melting temperature of P. falciparum (red), P. malariae (green), P. ovale (turquoise), P. vivax/ knowlesi (pink).
The box specifies the likely range of melting temperature variation. The melting temperature ranges were 63.6–63.9 for P. falciparum, 63.2–63.4 for P. malariae, 58.2–59 for P. ovale, 56.2–57.1 for P. vivax/ knowlesi. The figure was generated using R version 3.4.2 (2017-09-28) via RStudio version Version 1.1.383.
Table 2.
Crossing point (Cp) values of different concentrations of P. falciparum in the novel FRET-qPCR.
Fig 3.
Mixed infections of P. falciparum with P. vivax and P. falciparum with P. ovale.
Melting curves of amplicons post real-time PCR from mixed infections. Red: P. falciparum positive control; blue: negative control included reaction mixture and water; green: 10,000 P. falciparum/mL (uk 5083 1:100) + 20,000 P. ovale/mL (uk 5086); lilac: 10,000 P. falciparum/mL (uk 5083 1:100) + 6,400 P. vivax/mL (uk 5365).