Table 1.
Species records of Indochinese Macrobrachium prawns.
Fig 1.
Sampling localities of Macrobrachium in the Indochinese subregion.
Table 2.
Macrobrachium species samples used in this study including previously deposited sequences from BOLD.
Fig 2.
Representative morphology and live habitus coloration of five common Macrobrachium species; A. M. equidens B. M. niphanae C. M. sintangense D. M. dienbienphuense and E. M. lanchesteri. Approximate size shown in scale bar (5 mm).
Fig 3.
Ultrametric tree of Indochinese Macrobrachium indicating clade support level and species delimitation clustering results (part 1).
Abbreviations used on tree are as follows: Morpho, morphological identification; BIN, BIN method in BOLD; ABGD, automated barcode gap; bPTP, Bayesian Poisson tree processes; mPTP, multi-rate Poisson tree processes; GMYC, generalized mixed Yule coalescent model. Box colors indicate split (red) and lumped (blue) species recognized by each species delimitation method. Grey boxes indicate missing sequences. Morphological data from dataset in each delimitation method; sample ID with bold style indicates samples obtained from BOLD and NCBI. The horizontal line and species labels show the consensus result of species clustering.
Fig 4.
Ultrametric tree of Indochinese Macrobrachium indicating clade support level and species delimitation clustering results (part 2).
For abbreviations used on tree see Fig 3.
Fig 5.
Ultrametric tree of Indochinese Macrobrachium indicating clade support level and species delimitation clustering results (part 3).
For abbreviations used on tree see Fig 3.
Fig 6.
Results of genetic distance obtained from BOLD under K2-parameter model; A. Mean intra sequence divergence B. Distance to nearest-neighbor species based on sequence divergence C. Comparison of maximum intraspecific variation and distance to nearest-neighbor species D. Comparison of mean intraspecific variation and distance to nearest-neighbor species.
Table 3.
Summary of Mean-Max intraspecific variation in each Macrobrachium species and the distance to nearest-neighbor species.
Fig 7.
Results of barcoding gap representing intra- and inter-specific variations of Macrobrachium species retrieved from BOLD and this study.