Fig 1.
Colonic mucosa was examined using high-definition white light and virtual electronic chromoendoscopy demonstrating vascular and mucosal architecture of healing using a) iScan 1 b) iScan 2 c) iScan Optical Enhancement.
Fig 2.
Overview diagram demonstrating the process of sample acquisition and preparation for Raman Spectral measurement and data analysis.
Table 1.
Demographics of patients included in the analysis of mucosal healing vs. active inflammation.
Fig 3.
UC active inflammation (red) vs. UC mucosal healing (blue).
a) Self-organising Map (SOM) demonstrates the grouping. Each dot on the map represents a neuron, and each neuron represents a spectrum. Each new neuron will be drawn to its nearest vector, thereby causing clustering of neurons that have similar characteristics. The colour of the hexagon represents the heaviest weighting of neurons b) Self-organising Map Discriminant Index (SOMDI) providing a visual representation of the SOM data, highlighting the differences in spectra.
Table 2.
Relative intensity of Raman shifts between UC MH vs active inflammation taken from SOMDI.
Fig 4.
CD active inflammation (red) vs. MH (blue).
a) SOM demonstrates the groupings of MH vs active inflammation and b) SOMDI highlighting the spectral differences from the SOM.
Table 3.
Relative intensity of Raman shifts between CD healing vs non-healing taken from SOMDI.
Table 4.
Demographics of patients included in the exploratory analysis of pre- vs. post-biologic therapy and patients without IBD (healthy control).
Fig 5.
UC pre- (red) vs. post-biologic therapy (blue) vs. healthy control (black).
a) Self-organising Map (SOM) b) Self-organising Map Discriminant Index (SOMDI) highlighting the spectral differences from the SOM.
Table 5.
Relative intensity of Raman shifts between UC pre biologic and post biologic and HC taken from SOMDI.
Fig 6.
CD pre- (red) vs. post-biologic therapy (blue) vs. healthy control (black).
a) Self-organising Map (SOM) demonstrates the groupings and b) Self-organising Map Discriminant Index (SOMDI) highlights the spectral differences from the SOM.
Table 6.
Relative intensity of Raman shifts between CD pre-biologic and post-biologic and HC taken from SOMDI.