Fig 1.
CPE and IFA of McCoy cells after Siamese crocodile Chlamydia infection.
(A) McCoy cells were infected with Chlamydia at 30°C. (B) McCoy cells were infected with Chlamydia at 37°C. CPE of uninfected (control cells) and infected McCoy cells was observed by phase contrast microscopy at 32× magnification (scale bar = 10 μm). For the IFA test, infected McCoy cells were detected with Chlamydiaceae family-specific mouse monoclonal antibody and observed by fluorescence microscopy at 40× magnification (scale bar = 50 μm). CPE and IFA at each temperature were observed at 24, 48, 72, 96, and 120 hpi.
Table 1.
Comparison of incubation temperature for C. crocodili isolation in McCoy cells at 0, 24, 48, 72, 96, and 120 hpi by measuring DNA quantity.
Fig 2.
Electron microscopic images of McCoy cells infected with Siamese crocodile Chlamydia.
(A) Chlamydia accumulated in the cytoplasm of the infected McCoy cells. (B) Typical forms of Chlamydia were observed: EBs, RBs, and IBs.
Table 2.
Genome comparison of C. crocodili with its closely related species, C. poikilothermis and C. caviae.
Fig 3.
Phylogeny and pairwise amino acid sequence identity of nine taxonomic markers of other Chlamydia comparing with C. crocodili.
DnaA, SucA, Hyp325, and Fabl with cut-off values of ≥70%, ≥64%, ≥57%, and ≥78%, respectively, are used to delineate species of the same genus. RpoN, FtsK, PepF, Adk, and HemL with cut-off values of ≤96%, ≤98%, ≤96%, ≤95%, and ≤95%, respectively, are used for new species delineation.
Fig 4.
The ompA phylogeny.
Fig 5.
Phylogenetic tree of seven concatenated housekeeping genes.