Table 1.
Number of sequences of class A GPCRs in crustacean transcriptomes.
Table 2.
Number of sequences in class A GPCR subclasses in crustacean transcriptomes.
Fig 1.
Maximum likelihood phylogenetic tree of opsins in four decapod crustaceans and other species.
Colors represents five clades of visual and non-visual opsins. Maroon: Long wavelength-sensitive (LWS) opsins. Dark green: Medium wavelength-sensitive (MWS) opsins. Purple: D. melanogaster Rh7 homologues. Orange: Onychopsin-like molecules. Blue: Non-visual opsins including peropsins, pteropsins, neuropsins, and arthropsins. Colors representing decapod crustacean species: P. argus (red), H. americanus (green), P. clarkii (pink), C. sapidus (blue). The model of substitution is LG+F+I+G4 according to BIC as selected by ModelFinder. The root is drawn on the LWS opsin clade. Scale bar represents expected number of substitutions per site. Ultrafast bootstrap support values are color-coded and size-scaled from 55 to 100.
Fig 2.
Maximum likelihood phylogenetic tree of small-molecule receptors in four decapod crustaceans and other species.
Colors represent clades of sequences. Dark green: Dopamine and Dopamine/Ecdysteroid receptors; Orange: 5HT receptors; Teal: Acetylcholine receptors; Yellow: Tyramine receptors; Dark blue: Histamine receptors; Brown: Eicosanoid (prostaglandin) receptors (PGE); Light purple: Adenosine (Ado) receptors; Dark purple: Octopamine (Oct) receptors; Gray: Adrenergic receptors. Colors representing decapod crustacean species: P. argus (red), H. americanus (green), P. clarkii (pink), C. sapidus (blue). The model of substitution is LG+F+I+G4 according to BIC as selected by ModelFinder. Tree is unrooted and the root is drawn on the clade containing octopamine β receptors. Scale bar represents expected number of substitutions per site. Ultrafast bootstrap support values are color-coded and size-scaled from 36 to 100.
Fig 3.
Maximum likelihood phylogenetic tree of neuropeptide receptors, leucine-rich repeat-containing GPCRs, and orphan receptors.
Colors representing receptor types are neuropeptide receptors (dark green), leucine-rich repeat-containing GPCRs (brown), and orphan receptors (purple). Colors representing decapod crustacean species: P. argus (red), H. americanus (green), P. clarkii (pink), C. sapidus (blue). The model of substitution is LG+F+G4 according to BIC as selected by ModelFinder. Tree is unrooted and the root is drawn on the clade containing leucine-rich repeat-containing GPCRs. Scale bar represents expected number of substitutions per site. Ultrafast bootstrap support values are color-coded and size-scaled from 23 to 100.
Fig 4.
Maximum likelihood phylogenetic tree of sequences for all class A GPCRs in the four decapod crustacean species from this study.
Colors representing the receptor clades are: Small-molecule receptors (blue), Neuropeptide receptors (green), Opsins (orange), LGRs (brown), Orphan receptors (gray). Colors representing decapod crustacean species are: P. argus (red), H. americanus (green), P. clarkii (pink), C. sapidus (blue). The model of substitution is LG+F+I+G4 according to BIC as selected by ModelFinder. Tree is unrooted and the root is drawn on the opsin clade. Scale bar represents expected number of substitutions per site. Ultrafast bootstrap support values are color-coded and size-scaled from 15 to 100.
Table 3.
Class A GPCRs enriched in LF compared to brain.
Table 4.
Class A GPCR enriched in dactyl compared to brain.