Table 1.
Effect of anti-phosphotyrosine and anti-ACRBP antibodies upon sperm-ZP binding#.
Table 2.
Effect of anti-phosphotyrosine# and anti-ACRBP antibodies$ upon fertilization*.
Table 3.
Influence of anti-phosphotyrosine# and anti-ACRBP antibodies$ upon sperm capacitation and the AR$ $.
Table 4.
Impact of anti-ACRBP on the AR induced by thapsigargin and A23187#.
Table 5.
Effect of anti-ACRBP on the AR induction of boar sperm by solubilized ZP#.
Fig 1.
Indirect immunolocalization of SERCA 2 in fixed and permeabilized porcine sperm.
Panel A indicates that acrosomal fluorescent labelling is evident when detected with SERCA 2 polyclonal antibody. Panel B shows the phase contrast microscopy photograph of the same field. Panel C shows the non-specific fluorescence of sperm labeled only with secondary fluorescein-conjugated rabbit anti-goat antibody. The experiment was repeated three times and representative photos are shown.
Fig 2.
Indirect immunolocalization of ACRBP in fixed and permeabilized porcine sperm.
Panel A indicates acrosomal fluorescent labelling detected with anti-ACRBP antibody. Panel B shows the phase contrast microscopy photo of the same field (Panel B). The non-specific fluorescence of sperm labeled with secondary fluorescein-conjugated rabbit anti-goat antibody only is displayed in Panel C. The experiment was repeated three times and representative photos are shown.
Fig 3.
Western blots of sperm proteins incubated in capacitation medium with or without thapsigargin probed with anti-ACRBP antibodies.
Washed boar sperm were incubated for 0, 20, 30, 60 and 180 minutes in capacitation medium with or without thapsigargin. The blot represents results from three samples.
Fig 4.
Effect of sperm incubation in capacitation medium with thapsigargin, gingerol or BAPTA-K+ on the AR.
Washed porcine sperm were cultured in capacitation medium without any chemical modulators (control), or with thapsigargin, gingerol (Ging) or BAPTA-K+ (BAPTA) and stained with PSA-FITC to assess the AR (%). Different letters at 0, 20, 40, 60 and 180 minutes indicate significant differences as determined by one-way factorial analysis of variance with Fisher’s protected least significant difference (a-d: P < 0.05). The experiments were replicated three times. Data represent the mean percentages ± SD.