Fig 1.
The format of Illumina sequencing library (containing 2 variants) constructed by ChromInst.
Sequence of pre-amplification primers is gray shaded. The reverse-complimentary sequence of pre-amplification primer is shown in Bold. The sequences of exponential amplification primer 1 is underlined. The reverse-complimentary sequences of exponential amplification primer 2 is dash-underlined. The sequencing primer (Read 1 primer of Illumina) is indicated by the black arrow. The barcode is a sequence of hexamer nucleotides that is unique for each sample in a given sequencing run. The barcode sequencing primer is indicated by the dashed black arrow. The number 1 to 6 indicate the first 6 base position of a sequencing read, of which the signal emission is used for cluster recognition to initiate a sequencing run (N: either base of A, G, C, or T).
Table 1.
The primer designs.
Fig 2.
WGA-NGS library construction result from variable pre-amplification primer designs.
Fifty picograns human genomic DNA was used as amplification template. The amplification products are visualized on a 2% agarose gel as routine. M: DM2000 DNA Marker; Lane 1–5: the WGA products of design 1–5.
Table 2.
QC data of 15 single cells in a single run of Miseq sequencing.
Table 3.
The timeline of overnight PGT-A procedure in this work.
Fig 3.
Chromosomal CNV examination of embryo biopsy samples.
Chromosomes are aligned along X axis from 1 to 22 with X and Y at the end. Chromosomal copy number was set as Y axis. Aneuploidy was identified from 8 of the 14 embryos examined, which are as shown in the panel A: Sample 1; B: Sample 4; C: Sample 5; D: Sample 6; E: Sample 7; F: Sample 8; G: Sample 9; H: Sample13. Panel I shows a random example of normal diploid genome (Sample 11).
Table 4.
QC data of 14 embryo biopsy sequencing.