Fig 1.
Selection of 334 common DEGs from the three datasets (GSE3678, GSE33630, and GSE53157).
A. Venn diagram illustrating 136 upregulated DEGs (logFC≥1); B. Venn diagram illustrating 198 downregulated DEGs (logFC<1). The number denotes the number of genes shared between/among subtypes or the number of unique genes.
Table 1.
Screening of 334 commonly differentially expressed genes from three profile datasets, including 136 upregulated and 198 downregulated genes in PTC tissues, compared to normal thyroid tissues.
Fig 2.
The top 10 gene ontology (GO) and significantly enriched KEGG pathways.
A. BP; B. CC; C. MF; D. KEGG pathways. The X axis represents the enrichment levels; the Y axis represents the top 10 remarkably enriched categories. The color of the dot denotes the different P-value, and the size of the dot indicates the number of the candidate genes enriched in the GO and KEGG pathways.
Fig 3.
PPI network construction using STRING and Cytoscape.
Each node indicates a protein module; the edges represent protein interactions; red circles denote upregulated DEGs and blue circles represent downregulated DEGs.
Fig 4.
Module analysis by MCODE using Cytoscape.
A. module1; B. module2. Red circles represent upregulated DEGs and blue circles denote downregulated DEGs.
Table 2.
Selection of 30 central genes from PPI network, including 17 upregulated and 13 downregulated genes, by using the STRING and Cytoscape software.
Fig 5.
The association between the expression of the four hub genes and the overall survival of patients with PTC via GEPIA analysis.
Red curve denotes high expression level, while blue curve denotes low expression level.
Fig 6.
Analysis of hub gene expression levels with GEPIA analysis.
The transcript expression comparison of four genes in normal (n = 59) and tumor (n = 505) tissues via GEPIA.
Fig 7.
Analysis of hub gene expression levels with UALCAN analysis.
The transcript expression comparison of four genes in normal (n = 59) and tumor (n = 505) tissues via UALCAN.
Fig 8.
Representative IHC for LPAR5 expression in tissue microarrays (scale bar, 100 μm).
The categories of the expression of LAPR5 immunostaining in the papillary thyroid carcinoma at 100× magnification. Left: para-cancerous tissue; right: cross-section of a tumor obtained from PTC patient shows intense cytoplasmic staining.