Fig 1.
A) Prehatching (PRE) (stage 41 [2]), B) diapause III (DIA) (stage 43 [2]).
Fig 2.
Venn diagram showing proteins detected in prehatching (PRE) and diapause III (DIA). A protein was considered uniquely detected in PRE or DIA when it was identified in at least 2 replicates of one condition and was absent in the other. The number of proteins detected as uniquely present in DIA and PRE are shown light and dark gray, respectively. The overlapping section indicates the number of proteins common to both conditions.
Fig 3.
Volcano plot indicating proteins present in both sample sets but with a significant difference in relative abundance according to spectrum counts. Each spot represents one protein. In the y-axis, Log2 (p-value) is indicated. In the x-axis, Log2 (fold-change) is shown (negative values indicate proteins overrepresented in prehatching (PRE) samples, and positive values show proteins overrepresented in diapause III (DIA) samples). Blue dots correspond to proteins that show a significant difference in relative abundance between conditions. Proteins of interest are indicated.
Fig 4.
GO term enrichment of upregulated proteins in diapause III (DIA).
A) Biological process GO terms, B) molecular function GO terms, and C) cellular compartment GO terms. The color of each bar corresponds to the p-value of the Fisher test (see legend of each plot). The output of the analysis and the genes associated with each GO term are shown in S3 Table.
Fig 5.
GO term enrichment of upregulated proteins in prehatching (PRE).
A) Biological process GO terms, B) molecular function GO terms, and C) cellular compartment GO terms. The color of each bar corresponds to the p-value of the Fisher test (see legend of each plot). The output of the analysis and the genes associated with each GO term are shown in S3 Table.
Fig 6.
KEGG pathway analysis of upregulated proteins in prehatching (PRE) and diapause III (DIA).
Bars indicate the proportion of proteins upregulated in each condition in relation to the total number of proteins of the entire proteome assigned to each pathway (PRE: light blue, DIA: blue). On the left side, the upper hierarchical category is shown. See S4 Table for details.
Table 1.
KEGG enriched pathways in DIA and PRE.
Fig 7.
Brite classification of upregulated proteins in prehatching (PRE) and diapause III (DIA).
Bars indicate the proportion of proteins upregulated in each condition in relation to the total number of proteins of the entire proteome assigned to each category (PRE: light blue, DIA: blue). On the left side, the upper hierarchical category is shown.