Fig 1.
Mean heart rate before and after exposure to trail mucus, snail gel and water.
** indicates significant difference (p<0.05) using a repeated-measures t-test between base and test heart rate.
Fig 2.
Annotation of full-length secreted proteins identified in trail mucus of C. aspersum.
Yellow shading, cysteine residues; Blue shading, N-glycosylation sites; Green shading, signal sequences; Red shading, putative cleavage sites.
Fig 3.
Relative gene expression of secreted proteins in Foot (FT), Mucous Gland (MG), Central Nervous System (CNS) and Posterior (PT) and Anterior Tentacles (AT) of C. aspersum.
(A) Location of relevant snail tissues and abbreviations. (B) Relative gene expression based on z-score. Red indicates highest expression levels of gene as compared to other tissues. (C) Relative gene expression based on log2. Red indicates higher expression of gene as compared to other genes.
Fig 4.
Comparative analysis of perlucin-like proteins.
(A) Phylogenetic tree showing 6 clusters of perlucin-like proteins (colour-coded), including two found in Cornu aspersum (red cluster). (B) Multiple protein sequence alignment of mature perlucin-like proteins represented in phylogenetic cluster with Cornu aspersum. Shading and sequence logo (above alignment) provides level of conservation between species.
Fig 5.
Total ion chromatogram of mucus sample with the most frequently occurring compounds labelled.
Compounds are: 1 –styrene, 2 –benzaldehyde, 3 –decane, 4 –limonene, 5–2-ethylhexanol, 6 –nonanal, 7 –decanal, 8 –tetradecane, 9—propanoic acid, 2-methyl-1-(1,1-dimethylethyl)-2-methyl-3-propandiyl ester.
Fig 6.
Most abundant VOCs detected in C. aspersum trail mucus.