Fig 1.
Flow chart of the pigment quantification processes.
Table 1.
Concentrations of pigments in samples stored at different temperatures estimated using the GPS method in various wavelength ranges.
Table 2.
Calculated concentrations of total pigment in samples stored at different temperatures estimated using the GPS method in various wavelength ranges.
Table 3.
Variances in the estimated total pigment concentrations in samples stored at different temperatures according to different wavelength ranges.
Table 4.
P-values of the one-way ANOVA for differences in estimated total pigment concentrations between all wavelength ranges according to the storage temperature.
Table 5.
P-values of Levene’s test for homogeneity of variances in the estimated pigment concentrations (Allo to Dino) in all wavelength ranges according to the storage temperature.
Table 6.
P-values of Levene’s test for homogeneity of variances in the estimated pigment concentrations (Echin to Viola) and total concentrations in all wavelength ranges according to the storage temperature.
Table 7.
P-values of two-way ANOVA for differences in estimated pigment concentrations between wavelength ranges and storage temperatures respectively.
Table 8.
Concentrations of pigments (Allo to Fuco) in samples stored at different temperatures estimated using the GPS method with TLC in various wavelength ranges.
Table 9.
Concentrations of pigments (Lut to Viola) in samples stored at different temperatures estimated using the GPS method with TLC in various wavelength ranges.
Table 10.
Calculated concentrations of total pigment in samples stored at different temperatures estimated using the GPS method with TLC in various wavelength ranges.
Table 11.
P-values of the two-sample t-test for differences in estimated total pigment concentrations between storage temperatures.
Fig 2.
Concentrations of pigments estimated by the GPS method, and GPS method with TLC in samples stored at 20°C.
Interactive visualization of the data has been published online at https://www.ebi.ac.uk/biostudies/studies/S-BSST642. Allo = Alloxanthin; ββ-Car = β,β-Carotene; C.Neo = 9’-cis-Neoxanthin; Cantha = trans-Canthaxanthin; Chl.a = Chlorophyll a; Chl.b = Chlorophyll b; Chl.c1 = Chlorophyll c2; Chl.c2 = Chlorophyll c2; Diadino = trans-Diadinoxanthin; Diato = Diatoxanthin; Dino = Dinoxanthin; Echin = trans-Echinenone; Fuco = Fucoxanthin; Lut = Lutein; Myxo = Myxoxanthophyll; Peri = Peridinin; Phe.a = Pheophytin a; Phe.b = Pheophytin b; Viola = Violaxanthin.
Fig 3.
Concentrations of pigments estimated by the GPS method, and GPS method with TLC in samples stored at -20°C.
Interactive visualization of the data has been published online at https://www.ebi.ac.uk/biostudies/studies/S-BSST642. Allo = Alloxanthin; ββ-Car = β,β-Carotene; C.Neo = 9’-cis-Neoxanthin; Cantha = trans-Canthaxanthin; Chl.a = Chlorophyll a; Chl.b = Chlorophyll b; Chl.c1 = Chlorophyll c2; Chl.c2 = Chlorophyll c2; Diadino = trans-Diadinoxanthin; Diato = Diatoxanthin; Dino = Dinoxanthin; Echin = trans-Echinenone; Fuco = Fucoxanthin; Lut = Lutein; Myxo = Myxoxanthophyll; Peri = Peridinin; Phe.a = Pheophytin a; Phe.b = Pheophytin b; Viola = Violaxanthin.
Fig 4.
Concentrations of total pigment estimated by the GPS method, and GPS method with TLC in samples stored at 20°C.
Interactive visualization of the data has been published online at https://www.ebi.ac.uk/biostudies/studies/S-BSST642. Allo = Alloxanthin; ββ-Car = β,β-Carotene; C.Neo = 9’-cis-Neoxanthin; Cantha = trans-Canthaxanthin; Chl.a = Chlorophyll a; Chl.b = Chlorophyll b; Chl.c1 = Chlorophyll c2; Chl.c2 = Chlorophyll c2; Diadino = trans-Diadinoxanthin; Diato = Diatoxanthin; Dino = Dinoxanthin; Echin = trans-Echinenone; Fuco = Fucoxanthin; Lut = Lutein; Myxo = Myxoxanthophyll; Peri = Peridinin; Phe.a = Pheophytin a; Phe.b = Pheophytin b; Viola = Violaxanthin.
Fig 5.
Concentrations of total pigment estimated by the GPS method, and GPS method with TLC in samples stored at -20°C.
Interactive visualization of the data has been published online at https://www.ebi.ac.uk/biostudies/studies/S-BSST642. Allo = Alloxanthin; ββ-Car = β,β-Carotene; C.Neo = 9’-cis-Neoxanthin; Cantha = trans-Canthaxanthin; Chl.a = Chlorophyll a; Chl.b = Chlorophyll b; Chl.c1 = Chlorophyll c2; Chl.c2 = Chlorophyll c2; Diadino = trans-Diadinoxanthin; Diato = Diatoxanthin; Dino = Dinoxanthin; Echin = trans-Echinenone; Fuco = Fucoxanthin; Lut = Lutein; Myxo = Myxoxanthophyll; Peri = Peridinin; Phe.a = Pheophytin a; Phe.b = Pheophytin b; Viola = Violaxanthin.
Table 12.
P-values of the paired t-test for differences in estimated pigment concentrations at different temperatures before and after TLC was applied to the GPS method.
Table 13.
P-values of the paired t-test for differences in estimated total pigment concentrations at different temperatures before and after TLC was applied to the GPS method.