Fig 1.
Blood donor demographics of NYC metro area.
A; Choropleth of donation site locations used for collection of blood donor samples. Heatmap (gradient bar, top) corresponds to frequency of donors collected at each site. B; Distribution of NYC Metro area donor age (red bars) compared to NYC demographics (blue bars). Dotted lines represent best fit to a Gaussian distribution and r2 value represents calculated goodness of fit to distribution plot. C; Gender frequency of NYC Metro area donors (red bars) compared to NYC demographics (blue bars). Chi-square test for goodness of fit to observed (donors) versus expected (NYC demographics) results; * p<0.01. D; Ethnicity frequency of NYC Metro area donors (red bars) compared to NYC demographics (blue bars). Chi-square test for goodness of fit to observed (donors) versus expected (NYC demographics) results; * p<0.01.
Fig 2.
Serological and neutralizing activity analysis of NYC metro blood donors.
A; Frequency of NYC Metro area seropositive donors as determined using the Ortho Total Ig (yellow bar) or Abbott IgG (blue bar) HTSA assays. B; Venn diagram of donors determined to be seropositive using the Ortho (yellow) or Abbott (blue) HTSA assays. Seropositive donors that were reactive for both tests are indicated in overlap (green). C; Distribution of Ortho HTSA serological results between seropositive (red dots) and seronegative (blue dots) as determined by the Ortho HTSA assay. Median value and sample number is shown below graph. Dotted line shows S/co value (1.00 A.U.). D; Distribution of Abbott HTSA serological results between seropositive (red dots) and seronegative (blue dots) as determined by the Abbott HTSA assay. Median value and sample number is shown below graph. Dotted line shows S/co value (1.4 A.U.). E; Distribution of S1 ELISA serological results between seropositive (red dots) and seronegative (blue dots) as determined by either HTSA assay. Median value and sample number is shown below graph. Dotted line shows S/co value (100μg/mL). F; Distribution of NP ELISA serological results between seropositive (red dots) and seronegative (blue dots) as determined by either HTSA assay. Median value and sample number is shown below graph. Dotted line shows S/co value (100 μg/mL). G; Linear regression of seropositive donor of HTSA results. Dotted lines denote signal to cutoff (S/co) for each test and goodness of fit, r2, is shown. H; Spearman correlation coefficients, r, between each serological assay.
Table 1.
NYC metro seroprevalence estimation within demographic categories.
Table 2.
Correlation of serological results with neutralization activity.
Table 3.
Neutralization activity as a function of serological results.
Fig 3.
Correlation of NYC metro donor serological results with neutralization activity.
A; Frequency of Ortho HTSA (left) or Abbott HTSA (right) seropositive donor pseudovirus neutralization end-point titers. B-E; Box plots of seropositive donor serology results using the Ortho HTSA, Abbott HTSA, S1 ELISA and NP ELISA for each category of neutralization end point titers. Boxes and whiskers denote 1st and 3rd quartiles and range, respectively. Median serology value of each category is shown below graph.