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Fig 1.

Geographical location of the studied communities in the Darién region, Panama.

The Map was created using the software ArcGIS Desktop 10.7.1 and ArcGIS Online.

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Table 1.

Epidemiological information from the studied communities in Darién, 2016.

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Table 2.

Abundance and natural Plasmodium vivax infection in mosquitoes collected in Darién, Panama.

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Table 3.

Number of Anopheles species captured in the intradomicile and peridomicile in the communities sampled in the Darien Region, Panama.

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Fig 2.

Biting activity per hour of Anopheles (Nys.) darlingi in five communities of the Darien region, between March and November 2016 (geometric mean ± 95% confidence interval).

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Fig 3.

Biting activity per hour of Anopheles (Nys.) albimanus in five communities of the Darien region, between March and November 2016 (geometric mean ± 95% confidence interval).

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Fig 4.

Behavior of the monthly density of Anopheles (Nys.) darlingi in five communities of the Darien region, between March and November 2016 (geometric mean ± 95% confidence interval).

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Fig 5.

Behavior of the monthly density of Anopheles (Nys.) darlingi in five communities of the Darien region, between March and November 2016 (geometric mean ± 95% confidence interval).

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Table 4.

Number of Anopheles mosquito species captured with protective human-baited capture and CDC light traps per month in the communities under study in the Darien Region, Panama, 2016.

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Table 5.

Plasmodium vivax prevalence and positive pool numbers from An. (Nys.) albimanus and An. (Nys.) darlingi mosquitoes collected in Darien, Panama.

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Fig 6.

Nested PCR agarose gel electrophoresis to detect infection by Plasmodium spp. in pool of Anopheles spp. TM: DNA ladder (100bp, Qiagen), Pf: P. falciparum positive control (205 bp), Pv: P. vivax positive control (120 pb), Lane 1,3,6,8,9: pool samples of An. (Nys.) albimanus negative, lane 2,10,11,12,13: pool sample of An. (Nys.) darlingi negative, lane 4 and 5: pool sample An. (Nys.) darlingi positive for P. vivax, lane 7: pool sample An. (Nys.) albimanus positive for P. vivax, CN: negative control of PCR.

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Fig 7.

Phylogenetic analysis of P. vivax CSP gene.

The phylogeny tree was constructed with the neighbor-joining method with the JTT (John Taylor Torton) model using the MEGA 3 program.

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