Fig 1.
Unsupervised statistical analysis for all samples.
Principal component analysis of the five sample types. Dark Purple indicates the EDTA anticoagulated whole blood samples; light purple—EDTA plasma samples, light blue—citrate plasma; green—heparinized plasma, and orange—serum samples.
Table 1.
Number of significantly altered biochemicals in blood collected in different tube collection types.
Fig 2.
Differences in levels for several clinically relevant biochemicals.
(A)Box plot for serotonin. The boxes outline the 2nd and 3rd quartile (middle 50%) of the data for each sample type. (B) Box plot for bilirubin. (C), (D), and (E) Box plots for Adenosine, Guanosine, and Inosine, respectively. The error bars on the graph represent 1.5 × IQR (interquartile range for that metabolite within the sample type. The line within the box represents the median of the data and the “+” symbol represents the mean of the data set.
Table 2.
Heme, bilirubin, and bilirubin degradation products in whole blood, plasma and serum represented as fold-of-change compared to levels in EDTA plasma.
Table 3.
Fold-of-change in acylcarnitine levels in the blood specimens versus EDTA plasma.
Fig 3.
Box plots for biomarkers of kidney function.
Box plots for C-glycosyltryptophan, pseudouridine, N-acetylthreonine, creatinine, and phenylacetylglutamine are outlined. The boxes outline the 2nd and 3rd quartile (middle 50%) of the data for each sample type. The error bars on the graph represent 1.5 × IQR (interquartile range for that metabolite within the sample type. The line within the box represents the median of the data and the “+” symbol represents the mean of the data set. An “*” indicates a p<0.05 for the matched-pair comparison between the indicated sample types.
Fig 4.
Linear correlation plots for biomarkers of kidney function.
Scatter plots for C-glycosyltryptophan, pseudouridine, N-acetylthreonine, creatinine, and phenylacetylglutamine are outlined. The values for each metabolite were plotted for the serum and lithium heparin samples for all 27 donors in the study. The equation and R2 values for the best fit lines are outlined within each graph. The dotted lines indicate the 95% confidence interval for each linear fit.