Table 1.
Characteristics of study participants.
Table 2.
BAL fluid recovery, cell yield and cell differentials of LTBI and RSTR participants.
Fig 1.
A representative flow analysis for a) PBMC and b) BAL cells. CD56 NK percentage was quantified as a subset of the live CD3- population. Live CD3 lymphocytes were gated as shown to determine gamma delta, CD56 NKT, CD8, and CD4 subsets. CD45RA and CCR7 markers were then used to subset CD4 and CD8 cells into CM, EM, Naive and TEMRA groups.
Table 3.
CD3+ T cell subset and CD3- NK cell percentages in BAL and PBMC lymphocytes from LTBI and RSTR participants.
Fig 2.
CD4+ and CD8+ cell subset percentages based on CD45RA and CCR7 gating.
a) BAL lymphocyte subsets: N = 11 LTBI and14 RSTR, b) PBMC lymphocyte subsets: N = 13 LTBI and14 RSTR. PBMC CD8 TEMRA median percentage was larger in LTBI compared to RSTR (unadjusted P = 0.02/adjusted P = 0.034). Comparison of BAL vs PBMC: TEM median CD4+ and CD8+ percentages were larger in BAL compared to PBMC (P < 0.0001).