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Table 1.

Oligonucleotide primers and probes used in this work.

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Table 2.

Primer mismatches for Snow Mountain Virus used in this work.

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Fig 1.

Comparison of standard curves generated using a one-step real-time RT-PCR [11] and a nested real-time PCR [12].

Each curve was generated using four replicates for each dilution sample tested. Standard error bars are shown for each curve.

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Table 3.

Performance comparison of a one-step real-time RT-PCR and a nested real-time PCR for detection of GII.2 norovirus in fecal samples when DIG-labeled hybridization is used for amplicon confirmationa.

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Table 3 Expand