Fig 1.
Analysis of ortholog and co_orthologs present in Escherichia coli and Schizosaccharomyces pombe.
Venn diagram showing the distribution of shared orthologous clusters among Escherichia coli and Schizosaccharomyces pombe. Clusters of orthologs genes show that E. coli shares 343 clusters of orthologs genes with S. pombe, possessing 385 clusters that are only present in the bacteria and 436 in the fission yeast. Table shows the cluster of orthologs enriched in E. coli, S. pombe and in both organisms.
Fig 2.
Functional gene set enrichment analysis of GO, KEGG pathways and local network STRING (LNS).
DEGs data (RNA-seq) was analyzed for enriched gene sets of GO, KEGG pathways and local network STRING (LNS) terms using an FDR of 25% in the STRING database for all the genes of E. coli (Eco) (a) and S. pombe (Spo) (b) ranked by their Log2FC and separated by the condition where they were enriched (Eco_Glu_A25 and Spo_Glu_A25 in glucose and Eco_Gly_A25 and Spo_Gly_A25 in glycerol/acetate). (c) Same as in a but with a subset of ortholog and co_ortholog (O) genes from E. coli. (d) Same as in b but with a subset of ortholog and co_ortholog (O) genes from each S. pombe. (Eco_Glu_O25 and Spo_Glu_O25 in glucose and Eco_Gly_O25 and Spo_Gly_O25 in glycerol/acetate) Common terms in both conditions for E. coli and S. pombe are indicated in the tables.
Fig 3.
Relationship between Pearson’s correlation coefficient of log2 fold change of orthologs between E. coli and S. pombe.
Each point (x, y) represents a gene orthologous pair formed by log2FC value in E. coli and S. pombe. (a) Representation of global orthologs gene pair. (b) Selected point in Glycolysis/Gluconeogenesis pathway. (c) Selected point in TCA cycle pathway. (d) Selected point in pentose phosphate pathway. Negative slope of the function implies negative correlation or negative association. A positive correlation (positive slope) indicates direct association.
Fig 4.
Light microscopic detection of flagella in E. coli.
Bacteria were grown on YE medium supplemented with 2% glucose (a) or 2% glycerol and 0.2% sodium acetate (b and c), at 37° C for 2 h and stained with Leifson ’s stain. Cells growth in glycerol/acetate are shorter than in glucose and is evident in the peritrichous and polar flagella (arrows in c). Length bar, 5 μm.
Table 1.
GSEA analysis of KEGG metabolic pathways gene sets enriched in E. coli and S. pombe grown in glucose (GLU) and glycerol/acetate (GLY).
Fig 5.
Microscopic analysis of cells cultured in YE medium with two different carbon sources.
Wild-type S. pombe cells were incubated at 30°C to mid-log phase in YE containing 2% glucose (a) and YE medium containing 2% glycerol and 0.2% sodium acetate (b). Arrows in panel B mark cells displaying malformed and rounded shape. Length bar, 10 μm.