Table 1.
A. Components of high-speed RT-PCR mixtures (for the E gene).
B. Components of high-speed RT-PCR mixtures (for the RdRp Gene).
Table 2.
GeneSoC® primer and probe arrangement.
Table 3.
GeneSoC® PCR conditions.
Table 4.
LightCycler® 480 primer and probe arrangement.
Table 5.
LightCycler® 480 PCR conditions.
Fig 1.
Results of the measurement of SARS-CoV-2 RNA standards using the GeneSoC®.
E (A) and RdRp (B) Primers. The concentrations of the SARS-CoV-2 RNA standards were 5 × 101, 4 × 101, 3 × 101, 2 × 101, 1× 101, 5 × 100, and 2× 100 copies/μL. For 5 × 101, 4 × 101, 3 × 101, 2 × 101, and 1 × 101 copies/μL, a rising waveform was observed, and positive results were confirmed for both the E and RdRp primers. For 5 × 100 copies/μL, a rising waveform was only observed with the E primer. For 2× 100 copies/μL, no rising waveform could be identified with both E and RdRp primers. E primer (E), RdRp primer (RdRp).
Table 6.
Comparison of the primer detection sensitivity of GeneSoC® and LightCycler®.
Table 7.
Within-run reproducibility.
Table 8.
Between-run reproducibility.
Table 9.
Reproducibility of GeneSoC® among different detection units.
Table 10.
Comparison of clinical sample detection between GeneSoC® and LightCycler® 480.