Fig 1.
Phylogenetic tree of HAdV strains from children with acute respiratory infection in Buenos Aires, Argentina -2000 to 2018.
Buenos Aires samples are in red, named by the laboratory identification number followed by the isolation year. GenBank accession numbers of all available reference sequences (blue) are shown. Phylogenetic trees obtained by Maximum Likelihood methodology based on the HAdV partial hexon gene (HVR 1–6) by HAdV species. For each dataset the best fit model was used: I. TPMuf+Γ, II. TIM2+ Γ+I, III. TIM2+ Γ+I. The branch support was determined by bootstrapping (1000 pseudoreplica).
Table 1.
HAdV genotypes detected by year in children with acute respiratory infection in Buenos Aires, Argentina from 2000 to 2018.
Fig 2.
Phylogenetic analyses of HAdV genotypes C2 (I), C5 (II) and E4 (III).
Maximum likelihood phylogenetic trees for sequences from Argentina (red), and from other countries (black). The number between square brackets indicates how many additional HAdV sequences had the same position in the complete tree. The reference sequence(s) for each genotype are in blue. The best fit model was used according to Bayesian Information Criterion (I: HKY+I, II and III: HKY+Γ) for each tree. The numbers near the node represent the branch support (bootstrap % over 1000 pseudoreplica). Amino acid signature or particular features are described at the right of each cluster. I. AR Clade: sequences with an amino acid substitution E to K in the duplicated codon. III. Hollow bullets: sequence with a 4 amino acid deletion at positions 192 to 195.
Fig 3.
Sequence differences between HAdV-C2 clusters.
Differences at the sequenced region for HAdV C2, C89 and AR Clade are shown. For each position, the upper letters represent one-letter amino acid codes, and below the nucleotide triplet that encodes it. Letters in bold representing non-synonymous substitutions, also shown as dotted lines in the genome. Letters with light blue background represent synonymous substitutions. Type: Syn for synonymous and NoSys for non-synonymous substitutions. Question marks indicate sites that are singletons in AR Clade but are shared with C2.
Table 2.
Demographic and clinical characteristics of patients with ARI and a positive HAdV diagnosis in Buenos Aires, Argentina.