Fig 1.
Geographic distribution of the new species.
We also provide the geographic distribution of all clades labelled as Dendropsophus decipiens [2] and literature-based records of D. haddadi in the states of Alagoas [14,15], Bahia [16–20], Espírito Santo [17,21], Pernambuco [22,23] and Sergipe [24]. Municipalities: São Paulo State: (1) Cananéia; Rio de Janeiro State: (2) Seropédica; Minas Gerais State: (3) Santos Dumont, (4) Juiz de Fora, (5) Nanuque; Espírito Santo State: (6) Dores do Rio Preto, (7) Ibitirama, (8) Vitória, (9) Santa Teresa, (10) Aracruz, (11) Linhares, (12) Conceição da Barra (D. haddadi type locality); Bahia State: (13) Prado, (14) Camacan, (15) Ilhéus, (16) Aurelino Leal, (17) Itacaré, (18) Igrapiúna, (19) Jequié, (20) Mata de São João; Sergipe State: (21) Areia Branca; Alagoas State: (22) Maceió, (23) Maceió (Catolé and Ferrão Velho); Pernambuco State: (24) Lagoa dos Gatos, (25) São Lourenço da Mata (D. tapacurensis type locality), (26) Igarassu; Ceará State: (27) Guaramiranga.
Fig 2.
Holotype of Dendropsophus tapacurensis sp. nov. (CHP-UFRPE 5709).
(A) dorsal and (B) ventral views of body.
Fig 3.
Holotype of Dendropsophus tapacurensis sp. nov. (CHP-UFRPE 5709).
(A) Dorsal and (B) profile views of head. Views of (C) hand and (D) foot. View of female CHP-UFRPE 5703 (E) hand and (F) foot, evidencing the presence of pointed discs.
Fig 4.
Specimens of Dendropsophus tapacurensis sp. nov. depicting differences in pigmentation patterns and size.
Females (A) CHP-UFRPE 5712 and (B) CHP-UFRPE 5703; and males (C) CHP-UFRPE 5702 and (D) CHP-UFRPE 5713.
Fig 5.
Live specimens of Dendropsophus tapacurensis sp. nov. from type locality.
(A) adult male; (B) adult female; (C) amplectant pair; (D) egg mass deposited on leaves overhanging at standing water.
Table 1.
Measurement (in mm) for adult males and females of Dendropsophus tapacurensis sp. nov. type series.
Table 2.
Measurement (in mm) for adult males of species in the Dendropsophus decipiens Group.
Table 3.
Summary of acoustic parameters for species of the Dendropsophus decipiens Group.
Fig 6.
Advertisement call of Dendropsophus tapacurensis sp. nov.
(A) waveform of a call series with five notes (two seconds section), the note outlined is detailed in the (B, spectrogram) and (C, waveform; sound file: SCLEHP22) Air temperature 24.5°C and humidity 72%.
Fig 7.
Phylogenetic relationship of Dendropsophus tapacurensis sp. nov. based on the mitochondrial H1 dataset.
(A) Bayesian and (B) maximum likelihood inferences. Numbers below nodes indicate posterior probability (Bayesian) and bootstrap scores (maximum likelihood). Asterisk indicate posterior probability/bootstrap scores = 1.0/100. Scale indicates rate of base substitutions per site.
Table 4.
Tamura-Nei corrected pairwise distances (average p-values) in the Dendropsphus decipiens Group estimated from the final 400 bp of the 16S mitochondrial gene.
Fig 8.
Temporary ponds where we found the new species in calling activity.
(A) temporary pond within an Atlantic Forest strictly protected area; (B) temporary pond outside protected area amidst a sugar cane landscape.