Table 1.
The multiplex SARS CoV-2 RT-LAMP primer sets used in this study.
Fig 1.
Limit of detection for the monoplex SARS CoV-2 RT-LAMP assay.
The limit of detection for the monoplex SARS CoV-2 RdRP (A), E (B) and N (C) RT-LAMP assay was tested with synthetic RdRP, E and N plasmid ranging from 108 to 100 copies/μL, respectively. Numbers (1–10) indicated plasmid copy numbers/μL (1.0 × 108–1.0 × 100 copies/μL) and negative control (distilled water (DW) as non-template control).
Table 2.
Different concentration ratios of the SARS CoV-2 RdRP/E, RdRP/N, E/N and RdRP/E/N primer sets (1:1, 1:0.5 and 1:1.5, respectively) for the synthetic SARS CoV-2 RdRP, E and N gene plasmids.
Table 3.
Temperature gradient tests (60, 62 and 65°C) of the four multiplex combination RT-LAMP assay.
Fig 2.
Limit of detection (LOD) tests of the monoplex and multiplex RT-LAMP assay, Allplex™ 2019-nCoV Assay and PowerChek™ 2019-nCoV Real-time PCR kit for clinical SARS CoV-2 NP sample (range of 10−3–10−7).
(A) Detection limits of monoplex SARS CoV-2 RdRP (left), E (middle) and N (right) LAMP primer sets. (B) Detection limits of the multiplex SARS CoV-2 RdRP/E (upper left), RdRP/N (upper middle), E/N (upper right), RdRP/E/N (lower left) and RdRP/N/IC (lower middle) LAMP primer sets (C) Detection limits of Allplex™ 2019-nCoV assay (Left) and PowerChek™ 2019-nCoV Real-time PCR kit (Right).
Table 4.
Limit of detection (LOD) tests of the monoplex and multiplex RT-LAMP assay, Allplex™ 2019-nCoV Assay and PowerChek™ 2019-nCoV Real-time PCR kit for clinical SARS CoV-2 NP sample (range of 10−3–10−7).
Table 5.
Comparison of clinical performance of the multiplex SARS CoV-2 RT-LAMP assay with Allplex™ 2019-nCoV Assay and Powerchek™ 2019-nCoV Real-time PCR kit for SARS CoV-2 in clinical samples.
Table 6.
Cross-reactivity of the multiplex SARS CoV-2 RdRP/N/IC RT-LAMP assay with Allplex™ 2019-nCoV Assay and Powerchek™ 2019-nCoV Real-time PCR kit for SARS CoV-2 against other human infectious viruses.