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Table 1.

Clinical outcome measures collected during the clinical trial.

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Table 2.

Patient characteristics and presence of bacteriuria.

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Table 3.

Urinary cytokine concentrations of placebo and antibiotic treated women with refractory DO, with no growth or with bacteriuria.

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Fig 1.

In patients with bacteriuria, concentration of urinary cytokines in placebo (◇) and antibiotic (◆) treatment groups.

The concentration of urinary cytokines IL-1α (A), IL-1 receptor antagonist (ra) (B), IL-4 (C), IL-6 (D), IL-8 (E), IL-10 (F), CXCL10 (IP-10) (G), MCP-1 (H) and TNF-α (I) were measured by ELISA in women with bacteriuria (both UTI and polymicrobial infections) in both the antibiotic and placebo treatment groups. The cytokines IL-1α (A), IL-6 (D), IL-8 (E), IL-10 (F) and CXCL10 (G) were significantly lowered in the antibiotic treatment group in response to the presence of bacteriuria, compared placebo. In contrast, IL-4 (C) was significantly increased in the antibiotic treatment group compared to placebo. Data analysed using Mann-Whitney test and with the horizontal line across each column representing the median.

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Fig 2.

Longitudinal effect of antibiotics on urine cytokine concentrations.

The concentration of urinary cytokines were measured by ELISA and compared over time in the antibiotic (▲) and placebo (△) treatment groups (regardless of bacterial culture result). The concentrations of IL-1α (A), IL-6 (C) and IL-8 (D) were consistently significantly less in the antibiotic treatment group, compared to placebo. The concentration of IL-10 (E) was lower in the antibiotic treatment group than placebo, although this did not reach significance. The comparisons between the concentration of CXCL-10 (F) in the antibiotic and placebo treatment groups over time was more variable. Analysis was performed using a Holm-Sidak multiple comparisons t-test, where: ** = p<0.01, *** = p<0.001. Data presented as median and IQR.

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Table 4.

Correlations between cytokine concentrations and clinical outcome measures.

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Table 4 Expand