Table 1.
Direct clinical sample pools tested for SARS-CoV-2 RNA.
Table 2.
Nucleic Acid (NA) pooling design: Known positive individually extracted RNA and known negative samples separately extracted NA were pooled and a final volume of 5μl was taken from each pool to the master mix for amplification and detection.
Table 3.
A comparison of the influence of optimal pool size on test efficiency* when the disease prevalence rate is 0.05.
Fig 1.
Change in ct value of positive direct biological sample with low ct value (high viral copy) spiked with up to 9 negative samples for the two target genes (A N and B ORF 1ab genes).
Fig 2.
Change in ct value of RNA positive sample with low ct value (high viral copy) spiked with up to 9 negative samples for the two target genes (A N gene and B ORF1ab gene).
Fig 3.
Change in ct value of positive RNA sample with high ct value (low viral copy) spiked with up to 9 negative samples for the two target genes (A N gene and B ORF1ab gene).