Fig 1.
Process used to obtain the leaf and stem explants of L. ruthenicum.
Fig 2.
Process used to obtain L. ruthenicum samples of D and G groups used in MSAP analysis.
The abbreviations of plants are shown in brackets. Subscripts indicated the number of the plants used in the analysis.
Fig 3.
Plant regeneration of L. ruthenium from leaves and stems.
(A) Callus (circle) and adventitious buds (white arrow) derived from leaf explant (black arrow); (B) Rosette shoots from leaf callus; (C) The rooted plantlets from leaf explants; (D) Multiple shoots from axillary bud and nodular callus (black arrow) from cross section of stem explant; (E) The nodular callus (arrow) was magnified; (F) (G) Adventitious buds (arrow in F) from nodular callus of stem explant; (H) All the shoots from a single stem explant; (I) The rooted plantlets from stem explant; (J) Plantlets after acclimatization.
Table 1.
Effect of genetic background on callus, multiple shoot, adventitious bud and root induction in L. ruthenicum.
Table 2.
Cytosine methylation level of L. ruthenicum D group based on MSAP analysis using 14 primer pairs.
Table 3.
Cytosine methylation level of L. ruthenicum G group based on MSAP analysis using 14 primer pairs.
Table 4.
Changes in cytosine methylation pattern in the in vitro plants from leaf calli, stem calli and axillary buds compared with the corresponding in vitro donors of L. ruthenicum.
Table 5.
Changes in cytosine methylation pattern in the ex vitro plants regenerated from leaf calli and stem calli, and ex vitro plantlets derived from axillary buds compared with the corresponding ex vitro donors of L. ruthenicum.
Table 6.
Locus-specific MSV in ex vitro plants from axillary buds, stem calli and leaf calli compared with that of the corresponding in vitro plants of L. ruthenicum.
Fig 4.
Dendrogram illustrating coefficient similarities among samples in L. ruthenicum D group by the UPGMA cluster analysis based on the MSAP profiles (A), and associations among the samples in D group revealed by PCA (B).
Fig 5.
Dendrogram illustrating coefficient similarities among samples in L. ruthenicum G group by the UPGMA cluster analysis based on the MSAP profiles (A), and associations among the samples in G group revealed by PCA (B).