Fig 1.
Transversal sections (A–H) of a 21-day-old male rat fetus (Bouin’s fixation, Wilson’s free-hand method, with own modification for the head examination [22].
Fig 2.
Determining the Index of Length of Ossified Part (ILOP; 1) and Index of Width Of Ossified Part (IWOP; 2) of humerus and femur in Morphologically Normal Living Fetuses (MNLF) of M. musculus on gestation day (GD18; [24]; http://journal.itb.ac.id/index.php?li = article_detail&id = 625).
Table 1.
Results of soft tissue examination using the free hand razor blade technique through eight points of fetus body in Morphological Normal Live Fetus (MNLF) of M. musculus on Gestation Day (GD) 18 which previously were given 0.39 mg/kg bw LE3H through gavage on GD10(E1), injected with 5 mg/kg bw HgCl2 on GD9(E2), and administered 5 mg/g bw HgCl2 on GD9 and then 0.39 mg/kg bw LE3H on GD10(E3).
Meanwhile the controls (E0) were administered double-distilled water only.
Fig 3.
Results of soft tissue examination (see also Fig 1) using the freehand razor blade technique through eight points of fetus body on gestation day (GD) 18.
Description, sagittal section trough: A: Brain; B: Eye; C: Oral cavity and digestive tract; D: Esophagus and throat; E: Spine, lungs and heart; G: Small intestine and colon; n: normal anatomy; ad: defective anatomy; 1: normal hind brain; 2: small hind brain; 3: normal len vesicle; 4: small len vesicle; 5: normal respiratory tract; 6: narrow respiratory tract; 7: normal palate; 8: wavy palate; 9: normal throat channel; 10: narrow throat channel; 11: normal trachea; 12: narrow trachea; 13: normal small intestine; 14: narrow small intestine.
Fig 4.
Percentage normal and defective anatomy in Morphologically Normal Living Fetuses (MNLF) of M. musculus on Gestation Day (GD)18 which previously were given 0.39 mg/kg bw LE3H through gavage on GD10(E1), injected with 5 mg/kg bw HgCl2 on GD9(E2), and administered 5 mg/kg bw HgCl2 on GD9 and then 0.39 mg/g bw LE3H on GD10(E3).
Meanwhile the controls (E0) were administered double-distilled water only.
Table 2.
Results of soft tissue examination using the free hand razor blade technique through eight points of fetus body on Gestation Day (GD) which previously were given 0.39 mg/g bw LE3H through gavage on GD10 (E1), injected with 5 mg/kg bw HgCl2 on GD9 (E2), and administered 5 mg/kg bw HgCl2 on GD9 and then 0.39 mg/kg bw LE3H on GD10 (E3).
Meanwhile the controls (E0) were administered double-distilled water only.
Fig 5.
A sample of stained fetal bone with Alizarin Red S and Alcian Blue (the size of these images is disproportionate).
The dorsal stained fetal bones of four groups of experimental animals were observed to determine the number of ossified skulls, vertebrae, and ribs on gestation day (GD)18. The index of length of ossified part (ILOP, growth in length) and index of width of ossified part (IWOP, growth in diameter) of humerus and femur in morphologically normal living fetuses (MNLF) of M. musculus on GD18 were determined according to Fig 2.
Fig 6.
Index of length of ossified part (growth in length, ILOP) of humerus in M. musculus fetuses on Gestation Day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Table 3.
Length of humerus bone of M. musculus fetuses on Gestation Day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Fig 7.
Index of width of ossified part (growth in diameter, IWOP) of humerus in M. musculus fetuses on gestation day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Table 4.
Width of humerus bone of M. musculus fetuses on Gestation Day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Fig 8.
Index of length of ossified part (growth in length, ILOP) of femur in M. musculus fetuses on gestation day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Table 5.
Length of femur bone of M. musculus fetuses on Gestation Day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kgbw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Fig 9.
Index of width of ossified part (growth in diameter, IWOP) of femur in M. musculus fetuses on gestation day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.
Table 6.
Width of femur bone of M. musculus fetuses on Gestation Day (GD) 18 which previously were given 0.39 mg/g bw LE3H through gavage on GD 10 (E1), injected with 5 mg/kg bw HgCl2 on GD 9 (E2), and administered 5 mg/kg bw HgCl2 on GD 9 and then 0.39 mg/g bw LE3H on GD 10 (E3).
Meanwhile, the controls (E0) were administered double-distilled water only.