Table 1.
Fluorescence readings from anti-influenza FITC probes reveal the optimal bPEG2kSA concentration to be 10 μM.
Fig 1.
Capture coating schematic depicting the layers of the custom avidin-biotin complex adsorbed to optically polished, c-cut sapphire slide windows.
The hemagglutinin (HA) glycoproteins on the envelope of the Influenza virus bind sialic acid of the functionalized biotin-PEG linker (structural formula shown, insert, was provided by the supplier, Nanocs, Inc.).
Fig 2.
3D AFM topography images with corresponding 1D profiles through dashed lines as shown.
A) 460 μm sapphire c-cut slide. B) Capture coating on sapphire slide. C) Influenza A H3N2 virus cluster immobilized in capture coating on sapphire slide. The tendrils of the capture coating bPEG2kSA receptor tethers are clearly seen. D) A 2D example image of one of many single virions (this one Influenza A H3N2) immobilized in capture coating. 2DFFT analysis for all four AFM scans shown in S2 Fig.
Table 2.
Parameters characterizing surface features and roughness of samples from Fig 2.
Table 3.
Parameters and calculated values of capture efficiency (CEff) for each Influenza strain.