Fig 1.
Map showing the geographic location of Potočani (base map credit: USGS National Map Viewer, http://viewer.nationalmap.gov/viewer/).
Fig 2.
(A) The upper layers of the pit showing numerous commingled skeletons. (B) Schematics of the middle layer of the pit with different colors marking individual skeletons.
Fig 3.
Principal Component Analysis of the Potočani individuals and mixture proportions computed with qpAdm.
(A) Present-day West Eurasian individuals from the Human Origin dataset (small dots) were used to compute the principal components, with ancient individuals projected onto those coordinates. Potočani individuals with fewer than 50,000 recovered SNPs are displayed without black outline. (B) The model Anatolia Neolithic + Western hunter-gatherer was used to fit the ancestry of the Potočani individuals. The outgroup set was: Mota, Morocco Iberomaurusian, Villabruna, El Mirón, Eastern hunter-gatherers, Iran Neolithic, Levant Neolithic, Yamnaya Samara.
Fig 4.
Kinship relationships among the Potočani individuals.
We plot the kinship coefficient for each pairwise comparison.
Fig 5.
Pedigrees showing the kinship relationships among the Potočani individuals.
Circles and squares represent females and males, respectively. Different mitochondrial lineages are represented by different colors inside circles and squares, and different Y-chromosome lineages are represented by different colors inside circles on the upper left corner in males. Empty circles and squares represent individuals who are not closely related (3rd-degree or closer).
Fig 6.
Individual ROH in Potočani individuals and reference populations.
We plot ROH for each individual, summing all ROH in four length categories (stacked, colored bars). Individuals with the same population label are grouped into boxes. On the right, we depict a legend with expected ROH for (i) offspring of close kin in an outbred population (“recent loops”) and (ii) panmictic populations, using the values of Ringbauer and colleagues [35].