Fig 1.
The chemical structures of the seven plant hormones used in this study.
Fig 2.
Experimental workflow for extraction of phytohormones.
Fifty mg of tissues was extracted with 50% acetonitrile; it was then partially purified by RP-SPE. Dried residue was dissolved in 30% acetonitrile and was used for LC-MS/MS analysis.
Table 1.
Matrix effects in plant hormone determination.
Table 2.
A stable isotope-based quantification of plant hormones.
Table 3.
Quantification of plant hormones using standard addition.
Fig 3.
Comparison of standard addition method with stable isotope dilution method.
Plant hormone contents in the leaves (A), roots (B), and stems (C) were compared between standard addition and stable isotope addition methods. Significant differences were not observed.