Fig 1.
Visual comparison of two environmental water samples plated on 7H10 and 7H11 after acid treatment and RGM medium directly after six weeks of incubation.
The 7H10 and 7H11 plates grew many and varied microbial species, however RGM medium selected for only NTM A) white, beige colonies and B) white, beige and yellow-orange colonies.
Table 1.
Summary of positivity, overgrowth, and non-NTM Isolated on 7H10, 7H11, and RGM medium.
Table 2.
Identification of mycobacterial species on 7H10, 7H11, and RGM medium.
Fig 2.
Visual comparison of Purpureocillium lilacinum on 7H10, 7H11, and RGM medium after six weeks of incubation.
A) The 7H10 and 7H11 plates were overgrown with this fungus however the RGM medium partially inhibited the growth allowing recovery of NTM. B) Close up of inhibited growth of fungus from white box, black arrow shows the P. lilacinum colony. This was the only isolate of non-mycobacteria recovered from 223 environmental samples using RGM medium.
Table 3.
Identification of non-mycobacterial species on 7H10, 7H11, and RGM medium.
Table 4.
Comparison of known NTM strains on 7H10 and RGM medium.