Table 1.
Plasma biochemistry parameters.
Table 2.
Reference intervals for plasma biochemistry parameters of fasted sablefish.
Table 3.
Cell blood counts (CBC) were available from 72 animals, with the exception that thrombocyte counts were only available from 60 animals.
Fig 1.
a. Lymphocytes: Granular lymphocytes. Granular lymphocyte (GL), surrounded by rounder, basophilic immature erythrocytes (IE) that are smaller than the more mature erythrocytes (EC). Overall erythrocytes are in various stages of maturity contributing to a distinct polychromatic appearance, ranging from slightly eosinophilic to basophilic (Wright’s stain x100 objective). b. Lymphocytes: Mature lymphocyte and monocyte. Mature lymphocyte (L) and monocyte (M) with typical large oval eccentrically-positioned nucleus and a moderate amount of basophilic cytoplasm that contains three discrete cytoplasmic vacuoles. Mature lymphocytes are characterized as round cells with large, round nucleus with condensed chromatin and scant basophilic cytoplasm. Lymphocytes often display blebbing of the cell borders (Wright’s stain x100 objective). c. Lymphocytes: Reactive lymphocyte. Reactive lymphocyte (with Golgi body left to the nucleus, RL), small lymphocyte (L) with blebbing and neutrophil (N). Reactive lymphocytes are large cells, with a more open chromatin pattern and increased amounts of basophilic cytoplasm (Wright’s stain x100 objective).
Fig 2.
Neutrophils (N) are large leukocytes with large oval, clefted, or segmented nuclei and moderate to large amounts of slightly eosinophilic cytoplasm that often contains pale blue cytoplasmic inclusions consistent with Döhle bodies (Wright’s stain x100 objective).
Fig 3.
Neutrophil (N) and Monocyte (M).
Note similar size between these cells. Mature erythrocytes (EC) also identified (Wright’s stain x100 objective).
Fig 4.
Monocyte (M) contains several, variably sized, discrete cytoplasmic vacuoles and distinct, smooth cell borders, in contrast to the two lymphocytes (L) that display distinct blebbing. Also present is a thrombocyte (T) with a bi-lobbed nucleus (Wright’s stain x100 objective).
Fig 5.
Eosinophils (E) are slightly larger than mature lymphocytes (L) and have large, ovoid, eccentric nuclei and small amounts of cytoplasm that contains small, bright eosinophilic granules (Wright’s stain x100 objective).
Fig 6.
Thrombocytes (T) are found individually and in small aggregates in well-prepared blood smears. Thrombocytes are small and typically oval to elongate and commonly displaying nuclear pleomorphism with round to oval to reniform nuclei. The cytoplasm of thrombocytes is usually clear with occasional, small cytoplasmic granules (Wright’s stain x100 objective).