Fig 1.
Effects of P deficiency on the growth of C. grandis seedlings.
Fig 2.
Effects of P deficiency on the biomass of C. grandis.
Bars represent means ± SD (n = 8). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 3.
Effects of P deficiency on the contents of macronutrients in C. grandis leaves (A-F), stems (G-L) and roots (M-R).
Bars represent means ± SD (n = 3). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 4.
Effects of P deficiency on the contents of micronutrients in C. grandis leaves (A-E), stems (F-J) and roots (K-O).
Bars represent means ± SD (n = 3). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 5.
Effects of P deficiency on CO2 assimilation (A), stomatal conductance (B), and contents of intercellular CO2 (C), Chl a (D), Chl b (E) and Chl a+b (F).
Bars represent means ± SD (n = 5 for A-C and 3 for D-F). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 6.
Effects of P deficiency on the average of Chl a fluorescence (OJIP) transients (A); relative variable fluorescence between Fo to Fm, Vt = (Ft-Fo)/(Fm-Fo) (B) and Fo to F300 μs, Wk = (Ft-Fo)/(F300μs-Fo) (C); IP phase from I-step to P-step, IP phase = (Ft-Fo)/(FI-Fo)-1 = (Ft-FI)/(FI-Fo) (D), and the difference in Vt (△Vt, E) and Wk (△Wk, F) between P-deficient samples and control samples treated with 200 μM P in dark adapted leaves.
Data are represented as the means of six replicates.
Fig 7.
Effects of P deficiency on the production of H2O2 and the TBARS content in C. grandis leaves and roots.
Each bar represents mean ± SD (n = 4). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 8.
Effects of P deficiency on activities of antioxidant enzymes in C. grandis leaves.
Each bar represents mean ± SD (n = 4). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 9.
Effects of P deficiency on activities of antioxidant enzymes in C. grandis roots.
Each bar represents mean ± SD (n = 4). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.
Fig 10.
Effects of P deficiency on the contents of ASC, ASC+DHA, GSH, and GSH+GSSG, and on the values of ASC/(ASC+DHA) and GSH/(GSH+GSSG) in leaves (A-F) and roots (G-L) of C. grandis.
Each bar represents mean ± SD (n = 4). Differences among the treatments were analyzed by Student’s t-test. Different letters above the bars indicate a significant difference at P < 0.05.