Fig 1.
Representative picture of COMT gene PCR products.
The presence of 505 bp and 325 bp fragments on lanes 1, 9, 10, 13, 14 indicates the existence of homozygous Val/Val (G/G) allele, the presence of three fragments of 505 bp, 325 bp and 218 bp on lanes 2, 4, 5, 6, 7, 8, 11, 12 indicates the existence of heterozygous Val/Met (G/A) allele, while the presence of 505 bp and 218 bp on lanes 3, 15 16 indicates the homozygous Met/Met (A/A) allele. Lane 1 indicates a 100 bp DNA ladder (Thermo Fisher Scientific, Invitrogen DNA ladder 15628019).
Fig 2.
Representative picture of DRD4 gene PCR products.
The presence of both 549 bp and 429 bp fragments on lanes 3, 5, 6, 9, 12, 14, 16 indicates the existence of heterozygous 120 bp/240 bp allele, the presence of only 549 bp fragment on lanes 1, 2, 7, 10, 11, 15 indicates the existence of homozygous 240 bp/240 bp allele, while the presence of only 429 bp on lanes 4, 8, 13 indicates the homozygous 120 bp/120 bp allele. Lane 17 indicates a 100 bp DNA ladder.
Table 1.
Baseline characteristics of the study subject.
Table 2.
Behavioral and clinical characteristics of substance abused subject.
Table 3.
Genotype and allele distribution of COMT and DRD4 genes polymorphisms in substance abuser and control.
Table 4.
Association of genetic polymorphism of COMT and DRD4 genes with a risk of specific drug dependence.
Table 5.
Association of genetic polymorphisms of COMT and DRD4 genes with the age of onset of substance abuse.
Table 6.
Association of genetic polymorphisms of COMT and DRD4 genes with the pattern of drug habit.
Table 7.
Relationship of type of drug used with period of addiction.
Table 8.
Association of the genetic polymorphisms of COMT and DRD4 genes with a period of drug addiction to heroin abused subjects.