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Fig 1.

Study site location.

(A) Map of China. (B) Map of Fujian Province. (C) Map of Dehua county. The blue pentagram is the sampling area.

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Table 1.

Responses of physicochemical properties of soil at different depths to nitrogen addition in the Daiyun Mountain Nature Reserve in southeastern China.

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Table 2.

Effects of nitrogen deposition, soil layer, and their interactions on soil bacterial (B) and fungal (F) α diversity analyzed by two-way ANOVA.

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Fig 2.

Pearson’s correlation coefficients between soil properties and soil bacterial (A) and fungal (B) α-diversity.

α-diversity indices include Chao1, good coverage, observed species, PD whole tree, Shannon, and Simpson indices; SMC represents soil moisture content, CEC represents cation exchange capacity, SOC represents soil organic carbon, TN represents total nitrogen, TP represents total phosphorus, CN ratio represents the carbon:nitrogen ratio, and DOC represents dissolved organic carbon.

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Fig 3.

Relative abundance of dominant phyla of bacteria and fungi in the topsoil and subsoil under different N addition treatments.

Control treatment in topsoil is represented by CT_A, low nitrogen addition in topsoil is represented by LN_A, high nitrogen addition in topsoil is represented by HN_A; control treatment in subsoil is represented by CT_B, low nitrogen addition in subsoil is represented by LN_B, and high nitrogen addition in subsoil is represented by HN_B.

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Fig 4.

Redundancy discriminate analysis (RDA) plots illustrating the relationships between the dominant bacterial (A) and fungal (B) phyla and soil physicochemical properties and microbial biomass.

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Table 3.

Mantel test analysis of soil physicochemical properties and soil microbial biomass and community composition in topsoil and subsoil under different nitrogen addition treatments.

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