Fig 1.
Representative IHC staining (200×) of NUDT5 expression in 30 breast cancer specimens.
A–C, Very low NUDT5 expression in para-cancer tissues. D, Low NUDT5 expression in tumor tissues. E, Medium NUDT5 expression in tumor tissues. F, High NUDT5 expression in tumor tissues. The tissues of A and D, B and E, and C and F were obtained from each of 3 patients. G, NUDT5 protein expression was examined by IHC in 30 paired breast cancer and para-cancer tissues (P < 0.0001, Student’s t-test).
Table 1.
Patient and tumor characteristics.
Table 2.
NUDT5 expression in breast cancer and para-cancer tissues.
Fig 2.
NUDT5 expression in 140 breast cancer tissues and its prognostic value.
A, Representative IHC images showing the negative, low, and high expression of NUDT5 in breast cancer tissues (200×). B, Average IHC score of NUDT5 expression was examined in 84 breast cancer tissues (low expression) and 56 breast cancer tissues (high expression). C, Kaplan-Meier survival analysis according to IHC score of NUDT5 expression in patients with breast cancer (log-rank test).
Table 3.
Clinicopathologic findings and correlation with the NUDT5 expression (n = 140).
Table 4.
Univariate and multivariate analyses of the overall survival.
Fig 3.
NUDT5 knockdown reduces the proliferation of MCF-7, T47D, and MDA-MB-231 cells.
A, NUDT5 mRNA analysis with qRT-PCR. B, NUDT5 protein analysis with western blotting. C, Effects of NUDT5 knockdown on cell proliferation as detected by a CCK-8 assay. Student’s t-test, *P < 0.05.
Fig 4.
Low NUDT5 expression inhibits the migration and invasion of MCF-7, T47D, and MDA-MB-231 cells.
The invasion of the MCF-7, MDA-MB-231, and T47D cell lines was reduced by NUDT5 knockdown. The migration of the MCF-7 and MDA-MB-231 cell lines was inhibited while migration was not detected in the T47D cell line.
Fig 5.
The inhibition of AKT phosphorylation and Cyclin D1 expression in breast cancer cells by Knocking down NUDT5.
A, Representative images of MCF-7 and T47D cells with shNUDT5/empty vector (shScramble) and MDA-MB-231 cells with siNUDT5/siControl NUDT5. The expression of AKT, p-AKT, ERK, p-ERK, and cyclin D1 was analyzed by western blotting. B–D, Quantified expression of NUDT5, AKT, p-AKT, ERK, p-ERK, and cyclin D1 normalized to β-actin and scramble/negative control in MCF-7, T47D, and MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.
Fig 6.
Rescue NUDT5 expression reverse cell growth, migration, invasion ability and signaling pathway in MCF-7 cell.
Cell proliferation (A), invasion, migration (B) and activation of AKT and ERK (C) were all reinstated when re-expressing NUDT5 in NUDT5 knock down MCF-7 cell. Student’s t-test, *P < 0.05.