Fig 1.
Interpretation of results for COVID-PRESTO®.
COVID-PRESTO® uses anti-human immunoglobulin (Ig) M antibody (test line M), anti-human IgG antibody (test line G) and rabbit IgG (control line C) immobilized on a nitrocellulose strip. The Conjugate (recombinant COVID-19 antigens labeled with colloidal gold) is also integrated into the strip. A specimen is added to the sample well (S) followed by addition of assay buffer to the buffer well (B), IgM and/or IgG antibodies if present, will bind to COVID-19 conjugates forming antigen-antibody complexes. These complexes migrate through the nitrocellulose membrane by capillary action. When the complex meets the line of the corresponding immobilized antibody (anti-human IgM and/or anti-human IgG), the complex is trapped forming a burgundy colored band which confirms a reactive test result. The absence of a colored band in the test region indicates a non-reactive test result. To serve as a procedural control, a colored line always changes from blue to red in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred. If the control line does not switch colors, then the test is deemed invalid.
Table 1.
Instructions for use.
Table 2.
Satisfaction questionnaire.
Table 3.
Supervisor’s feedback.
Table 4.
Demographic characteristics of the study population.
Table 5.
Instructions’ comprehension.
Fig 2.
User satisfaction regarding the use of COVID-PRESTO® as a self-test.
Percentages of participants for each choice of response to the question no.7 of the satisfaction questionnaire.
Fig 3.
Participants with an education level 4 or 5 requested assistance more frequently.
Number of participants having asked for a supervisor’s assistance according to education level (* χ2 p<0.05 vs. education level 3 and below or level 6 and above).
Fig 4.
Description of the execution ratings according to the supervisors.
Number of participants in each rating category according to age (A) or education level (B).
Fig 5.
Description of the readability of the test results according to the participants.
Number of participants in each rating category according to age (A) or education level (B).