Table 1.
The primers used in the research.
Table 2.
The PCR amplification procedure of primers for the research.
Fig 1.
Root symptom of diseased Vitis vinifera.
(A) and (B) all show the root symptom of diseased Vitis vinifera, the arrow of fig show eggs of root-knot nematodes.
Table 3.
Morphometrics of Meloidogyne vitis sp. nov.
Fig 2.
Line drawings of Meloidogyne vitis sp. nov.
Male (A-F) A: Entire body of male; B, C: Anterior region of male; D, E: Tail region of male; F: Lateral field of male. Second-stage juveniles (G-I) G: Entire body of second-stage juveniles; H: Anterior region of second-stage juveniles; I: Tail region of second-stage juveniles. Female (J-O) J: Anterior region of female; K, L: Entire body of female; M: Eggs of female; N, O: Perineal pattern of female. (Scale bars: A, K, L = 200 μm; B-E, I, H, G, M-O = 20 μm; G = 100 μm; F = 10 μm).
Fig 3.
Light micrographs of Meloidogyne vitis sp. nov.
Male (A-D) A: Entire body of male; B: Anterior region of male; C, D: Tail region of male. Female (E-J) E: Entire body of female; F: Anterior region of female; G: Partial region of female; H, I: Perineal pattern of female; J: Eggs of female. Second-stage juveniles (K-O) K, O: Tail region of second-stage juveniles; L: Entire body of second-stage juveniles; M, N: Anterior region of second-stage juveniles (Scale bars: A, E = 200 μm; B-D, F, H, I, J, K, M-O = 20 μm; G, L = 100 μm).
Fig 4.
Scanning electron microscope photographs of Meloidogyne vitis sp. nov.
Female (A-E) A: anterior end in lateral view; B: anterior end in face view; C: perineal pattern; D: anterior end in lateral view and see the stylet; E: excretory pore. Second-stage juvenile (F-I) F: anterior end in lateral view; G: anterior end in face view; H: lateral field; I: tail region. Male (J-O) J: lateral field; K: tail region; L: anterior end in lateral view; M: anterior end in face view; N: excretory pore; O: tail region. (Scale bars: A, C, O = 20 μm; B, D, M = 3 μm; F, G = 2 μm; E, L, H, N = 5 μm; J, I, K = 10 μm).
Fig 5.
Malate dehydrogenase and esterase phenotype patterns obtained with electrophoresis of protein homogenates from five young egg-laying females of Meloidogyne vitis sp. nov. (lane Mv) and five young egg-laying females of the Meloidogyne javanica reference population (lane Mj).
(A) Malate dehydrogenase patterns. (B) esterase patterns. (C) Relative mobility (Rm) of malate dehydrogenase bands. (D) Relative mobility (Rm) of esterase bands.
Fig 6.
PCR electropherogram for different fragments of Meloidogyne vitis sp. nov. and Meloidogyne mali.
M: 2000 DNA marker; CK: The negative control consisting of water; Lanes 1–2: The ITS1-5.8S-ITS2 region of Meloidogyne vitis sp. nov. and Meloidogyne mali, respectively; Lanes 3–4: The D2/D3 region of Meloidogyne vitis sp. nov. and Meloidogyne mali, respectively; Lanes 5–6: The coxI region of Meloidogyne vitis sp. nov. and Meloidogyne mali, respectively; Lane 7: The coxII region of Meloidogyne vitis sp. nov.
Fig 7.
Phylogenetic relationships of Meloidogyne vitis sp. nov. with other root-knot nematodes based on ITS1-5.8S-ITS2 sequences.
Numbers to the left of the branches are bootstrap values for 1000 replications.
Fig 8.
Phylogenetic relationships of Meloidogyne vitis sp. nov. with other root-knot nematodes based on D2/D3 sequences of 28S rDNA.
Numbers to the left of the branches are bootstrap values for 1000 replications.
Fig 9.
Phylogenetic relationships of Meloidogyne vitis sp. nov. with other root-knot nematodes based on coxI-rRNA genes sequences.
Numbers to the left of the branches are bootstrap values for 1000 replications.
Fig 10.
Phylogenetic relationships of Meloidogyne vitis sp. nov. with other root-knot nematodes based on coxII-16S rRNA genes sequences.
Numbers to the left of the branches are bootstrap values for 1000 replications.
Fig 11.
PCR amplification of the supplied RKNs with the Mv-F/Mv-R primers.
M: 2000 DNA marker; CK: The negative control consisting of water. A: Lanes 1–6, The ITS1-5.8S-ITS2 region of Meloidogyne vitis sp. nov., Meloidogyne incognita, Meloidogyne javanica, Meloidogyne arenaria, Meloidogyne hapla and Meloidogyne enterolobii, respectively. B: Lanes 7–12, The amplification results of root-knot nematode species-specific PCRs of Meloidogyne vitis sp. nov., Meloidogyne incognita, Meloidogyne javanica, Meloidogyne arenaria, Meloidogyne hapla and Meloidogyne enterolobii, respectively.