Fig 1.
Degradation pattern of bromazepam (BMZ) by acidic and alkaline hydrolysis forming the degradate (ABP).
Fig 2.
HPLC chromatogram of 12 μg mL-1 of BMZ and 12 μg mL-1 of ABP (Rt = 4.397, 5.317 min respectively).
Mobile phase: Methanol: Water (70:30 v/v). Detection: DAD at 230 nm.
Table 1.
Validation parameters assay results of the proposed RP-HPLC method for the determination of BMZ and ABP.
Table 2.
Determination of BMZ in pharmaceutical formulations by the proposed RP-HPLC method and application of the standard addition technique.
Table 3.
Results of accuracy for the determination of BMZ and ABP by the proposed RP-HPLC method.
Table 4.
Experimental results of robustness testing for the determination of BMZ by the proposed RP-HPLC method.
Table 5.
System suitability parameters of the proposed HPLC method for the determination of BMZ and ABP.
Table 6.
Statistical analysis of the current RP-HPLC method and the published HPLC methodology for the determination of BMZ in dosage forms.