Fig 1.
Commonly examined post-occlusive reactive hyperemia parameters shown in a representative PORH response.
Resting flow (RF), maximal flow (MF), time to maximal flow (TMF), amplitude of the MF normalized to RF and BZ (AMPRF and AMPBZ) and 4-min post cuff-deflation area under hyperemic curve (4-min AUC) normalized to zero perfusion are shown. Time = 0 corresponds to cuff deflation. Magnified tracing on the top right shows the hyperemic response 30s post-cuff deflation.
Fig 2.
Normalization methods for the area under the curve (AUC).
The AUC subtracted from biological zero (AMPBZ) or subtracted from resting flow (AMPRF).
Fig 3.
Piecewise overshoot rate of change.
5s intervals from 5-20s (ORC5s,10s,15s,20s) hyperemic response illustrated in this tracing obtained from experimental data. Time = 0 corresponds to cuff deflation.
Table 1.
PORH parameters and their mathematical expressions.
Table 2.
Descriptive and reproducibility statistics for commonly used post-occlusive reactive hyperemia parameters.
Fig 4.
Average TMF (19.1 ± 4.9s) of the three PORH responses (VA, VB1, VB2).
These data were normally distributed. The skewness and kurtosis were -0.064 and -0.642, respectively.
Table 3.
Descriptive and reproducibility statistics for inter- and intra-day novel PORH parameters.