Fig 1.
Collected Malayan snail-eating turtle (Malayemys macrocephala) with Placobdelloides siamensis on the plastron (red arrows).
Fig 2.
Life cycle of Placobdelloides siamensis in the laboratory: gestation period (eggs in ovisacs on the (A) second day (white arrow), (B) third day (white arrow), and (C) fourth day (white arrow) after copulation); (D) a clutch of eggs attached to the substrate (pottery) (white arrow); (E) incubation period (eggs on the sixth day after deposition); (F) parental care period (broods on the (F) second day and (G) seventh day after hatching); and (H) maturation period (juveniles in the third week after hatching).
Fig 3.
Scanning electron microscopy of Placobdelloides siamensis: (A) eggs on the first day after deposition, (B) brood with annulation and nearly hatching eggs (white arrow) on the seventh day after deposition, and brood on the ventral surface of their parents on the (C) second day and (D) seventh day after hatching.
Fig 4.
Placobdelloides siamensis brood (with transparent bodies, red eyes (red arrows), and yolks (white arrows)) on the ventral surface of their parents on the second day after hatching.
Fig 5.
Mortality rate of Placobdelloides siamensis after feeding on and separating from Malayemys macrocephala from 25 July 2019 through 3 January 2020.
Fig 6.
Life cycle of Placobdelloides siamensis.
Table 1.
Life history data (day) of ten Placobdelloides siamensis individuals maintained in a laboratory at 26.5–34.0°C with an oxygen pump and host turtle (Malayemys macrocephala).
Table 2.
Comparison of glossiphoniid leeches in laboratories, including their host; gestation, incubation, parental care, and maturation periods (days); clutch sizes (eggs/clutch); and water temperature (°C).