Table 1.
Levels and codes of extraction variables used in BBD.
Table 2.
BBD matrix and its results for the optimization of UACE process.
Fig 1.
Effects of different extraction parameters on the yield of polysaccharides and triterpenoids of G. lucidum, as well as on the D value.
Open squares, polysaccharide yield; open triangles, triterpenoid yield; and filled circles, D value.
Table 3.
Analysis of variance for the fitted response surface quadratic model.
Fig 2.
Response surface plots showing the effects of extraction parameters on the D values in the extraction of polysaccharides and triterpenoids from G. lucidum.
A, effect of ultrasonic power and extraction temperature; B, effect of ultrasonic power and liquid/solid ratio; C, effect of ultrasonic power and extraction time; D, effect of extraction temperature and liquid/solid ratio; E, effect of extraction temperature and extraction time; and F, effect of liquid/solid ratio and extraction time.
Fig 3.
DPPH radical scavenging activity of the polysaccharides (A) and triterpenoid-rich extracts (B) from optimal UACE and traditional extraction methods. Filled circles, polysaccharides or triterpenoid-rich extracts from optimal UACE; open circles, polysaccharides from HWE or triterpenoid-rich extracts from ethanol maceration, and filled squares, BHT.
Fig 4.
Reducing power of the polysaccharides (A) and triterpenoid-rich extracts (B) from optimal UACE and conventional methods. Filled circles, polysaccharides or triterpenoid-rich extracts from optimal UACE; open circles, polysaccharides from HWE or triterpenoid-rich extracts from ethanol maceration; and filled squares, BHT.