Table 1.
The number and density of NGG-PAM and NAG-PAM sites on pepper chromosomes.
Fig 1.
Composition of pepper PAM sites.
A, NGG-PAM; B, NAG-PAM.
Fig 2.
Distribution of different kinds of CRISPR/Cas9 editing sites in the pepper genome.
A, NGG-PAM+NAG-PAM site; B, NGG-PAM site; C, NAG-PAM site; D, Class 0.0; E, Class 0.1; F, Class 1.0; G, Class 1.1; H, Class 2.
Fig 3.
Correlation between the number of CRISPR/Cas9 editing sites and chromosome length in pepper.
Fig 4.
Comparison of the number and density of CRISPR/Cas9 editing sites between genic and intergenic regions.
A, NGG-PAM+NAG-PAM site; B, NGG-PAM site; C, NAG-PAM site; D, Class 0.0+Class 1.0; E, Class 0.0; F, Class 1.0.
Table 2.
The number of CRISPR/Cas9 editing sites in different genomic regions.
Table 3.
The number of NGG-PAM sites with differences in specificity on pepper chromosome.
Fig 5.
PCR amplification of 19 highly-specific target sites.
M, DL2000 plus, 1 to 10 represent A1 to A10 belonging to class0.0; 11 to 19 represent B1 to B9 belonging to class 1.0 (S1 Table).
Table 4.
The number of highly specific NGG-PAM sites in different genomic regions.
Fig 6.
Histogram plots of gene numbers according to the number of exon-targeted highly specific NGG-PAM sites.