Table 1.
Performance measures of the four supervised learning methods for three-class classification.
Table 2.
Performance measures of the four supervised learning methods for two-class classification.
Fig 1.
Boxplots of normalized transcript count by Wooden Breast status.
Figure illustrates the expression pattern of 12 out of the 20 genes with a non-zero coefficient for the partially affected category (first row) and markedly affected category (second row) in LASSO or ENET analyses. ACE (angiotensin I converting enzyme); DGAT2 (diacylglycerol O-acyltransferase 2); ESM1 (endothelial cell specific molecule 1); HS3ST2 (heparan sulfate-glucosamine 3-sulfotransferase 2); LPL (lipoprotein lipase); LRP11 (LDL receptor related protein 11); NXPH2 (neurexophilin 2); MMP1 (matrix metallopeptidase 1); ROBO1 (roundabout guidance receptor 1); STMN2 (stathmin 2); TLR2-2 (toll like receptor 2 type 2) TLR4 (toll like receptor 4).
Fig 2.
Transmission electron microscopy micrographs of portions of vascular endothelial cell layer (En) from the pectoralis major (p. major) muscle.
(a) Unaffected chicken and (b) partially affected chicken. Compared to the relatively thinner endothelial cell layer from the unaffected chicken, the vascular endothelial layer from p. major muscles of Wooden Breast-affected chicken appears thicker with prominent and numerous organelles including mitochondria (M), ribosomes and rough endoplasmic reticulum (rER), membrane bound multivesicular body (open arrowhead), Weibel-Palade bodies (WPb) and a conspicuous tight junction (arrow). The vascular endothelial cells in the unaffected muscle depict a quiescent phenotype while those in the Wooden Breast-affected muscle presents an activated biosynthetic phenotype. Also shown are the intact myofiber (My) and myofiber nucleus (My-nu) in unaffected muscle, erythrocytes (Ery) in the blood vascular lumen (Ln), and collagen fibers (Cf) in the extracellular matrix indicating fibrosis in Wooden Breast-affected muscle.