Table 1.
Summary of sample numbers from both informal and commercial abattoirs.
Fig 1.
All the samples were first enriched then cultured on both TBX and CHROMagar plates. Biochemical test was then done on positive bacterial colonies and PCR was used to confirm STEC done on the positive presumptive bacterial samples using specific primers.
Table 2.
List of primer sequences against which the samples have been tested.
Fig 2.
Bacteria culture of E. coli and STEC from both informal and commercial abattoirs.
(A) E. coli cultures appearing blue-green on TBX plate. (B) STEC cultures showing typical mauve colonies on CHROMagar STEC plate.
Fig 3.
Presumptive E. coli and STEC positive samples based on growth on TBX and CHROMagar.
(A) Illustrating 44.6% of samples from abattoir A, 37.25% from abattoir B and 13.72% from abattoir C that showed E.coli growth on TBX. (B) Illustrating 10.58% of meat samples from abattoir A, 63.53% from abattoir B and 25.88% from abattoir C that showed STEC cultures (mauve) on CHROMagar STEC. (C) Indicating the presence of E.coli in 13.51% equipment swabs from abattoir B and 16.21% equipment swabs from abattoir C. (D) showing the presence of STEC in 5.40% equipment swabs from abattoir C.
Table 3.
Summary of detected STEC virulence genes from the meat sample bacterial isolates from informal and commercial abattoirs.
Table 4.
Characterisation of STECs from meat sample bacterial isolates from both abattoirs.