Table 1.
Mixed model ANOVA analysis of Shannon Diversity Index, and Chao1 in skin of diploid and triploid salmon.
Table 2.
Mixed model ANOVA analysis of Shannon Diversity Index, and Chao1 in gills of diploid and triploid salmon.
Fig 1.
Microbial composition in the skin of triploid and diploid Atlantic salmon at the steady state.
(A) Relative abundance at the phylum level for triploid and diploid skin at day 0. (B) Relative abundance at the genus level for triploid and diploid skin at day 0.
Fig 2.
Relative microbial composition in the gills of triploid and diploid Atlantic salmon at the steady state.
(A) Relative abundance at the phylum level for triploid and diploid gills at day 0. (B) Relative abundance at the genus level for triploid and diploid gills at day 0.
Table 3.
SAV3 levels in heart tissue samples from fish used in this study.
SAV3 titers were estimated using qPCR on nsP1 gene. ND—not detected (below detection limit).
Fig 3.
Alpha diversity metrics for the skin and gill microbiome of triploid and diploid control and infected Atlantic salmon.
(A) Total number of observed ASVs in the skin. (B) Shannon’s diversity index in the skin. (C) Chao1 index in the skin. (D) Total number of observed ASVs in the gills. (E) Shannon’s diversity index in the gills. (F) Chao1 index in the gills.
Fig 4.
Relative microbial composition in the skin of Atlantic salmon following challenge with SAV3.
Relative abundance at the genus level for triploid and diploid skin at day 0, day 21 unchallenged, and day 21 post-immersion in SAV bath challenge.
Fig 5.
Relative microbial composition in the gills of Atlantic salmon following challenge with SAV3.
Relative abundance at the genus level for triploid and diploid gills at day 0, day 21 unchallenged, and day 21 post-immersion in SAV bath challenge.
Fig 6.
Mean Candidatus Branchiomonas Ct values in gill samples of diploid and triploid Atlantic salmon as determined by RT-qPCR.
*** indicates P-values<0.001 by Paired Student’s T-test.
Fig 7.
Histological examination of the gills of triploid and diploid Atlantic salmon.
(A, B) Hematoxylin & eosin (H&E) stain of diploid and triploid day 0 control gill. (C, D) H&E stain of diploid and triploid day 21 control gill. (E, F) H&E stain of diploid and triploid day 21 SAV infected salmon gill. Arrows indicate epitheliocystis; arrowheads indicate thickening of secondary lamellae tips; double arrows indicate enlargement of basal region of lamellae; asterisks indicate fusion of secondary lamellae. Images are representative of N = 3/group. Scale bar: 250 μm.