Fig 1.
Chemical structure of acteoside, and its 1H-qNMR spectrum in methanol-d4 containing DSS-d6.
IS, internal standard (DSS-d6).
Fig 2.
Chemical structure of pedaliin, and its 1H-qNMR spectrum in DMSO-d6 containing DSS-d6.
IS, internal standard (DSS-d6).
Fig 3.
Chemical structure of MHB, and its 1H-qNMR spectrum in DMSO-d6 containing DSS-d6.
IS, internal standard (DSS-d6).
Fig 4.
Calibration curves for acteoside (a), pedaliin (b), and MHB (c). The calibration curve slopes were: acteoside, 4671.9; pedaliin, 14431; MHB, 6428. The resulting RMS values relative to MHB were 0.727 (4671.9/6428.0) for acteoside and 2.25 (14431/6428.0) for pedaliin.
Table 1.
Regression data of acteoside, pedaliin, and MHB.
Fig 5.
Representative HPLC chromatograms of each samples at 340 nm.
Dried sesame leaf powder 1, (b) Dried sesame leaf powder 2, (c) Dried sesame leaf powder 3, (d) Green juice powder 1, (e) Green juice powder 2. The peaks labeled 1 and 2 correspond to acteoside (tR: 14.1 min) and pedaliin (tR: 15.0 min), respectively.
Fig 6.
Representative HPLC chromatograms of a MHB (tR: 18.2 min) standard solution (100 μmol/L) at 254 nm.
Table 2.
Comparison of the acteoside and pedaliin contents in samples determined by two methods.