Table 1.
Top ten genes upregulated by 20% IQOS CSE at 6 h after exposure.
Fig 1.
Nuclear translocalization of NRF2 in rat ATI-like cells is detected using immunocytofluorescence at 0.5, 1, 2 and 4 h after stimulation with 20% IQOS CSE.
NRF2 (red), DAPI (blue).
Fig 2.
The expression levels of Hmox-1, Gsta1, Gsta3 and Nqo1 mRNA in rat ATI-like cells are upregulated at 6 h after stimulation with 10% and/or 20% IQOS CSE.
Only Gsta1 mRNA expression is upregulated at 24 h after stimulation with 20% IQOS CSE. Data represent results from three independent experiments. Each experiment was done in duplicate or triplicate wells. *: p < 0.05, ***: p < 0.001.
Fig 3.
HMOX-1 and NQO1 but not GSTA1 and GSTA3 proteins in rat ATI-like cells are induced at 48 h after 10% and/or 20% IQOS CSE stimulation.
A) representative blot, B) quantitation of HMOX-1, GSTA1, GSTA3 and NQO1 relative protein expression. Data represent results from three independent experiments. *: p < 0.05, **: p < 0.01, ***: p < 0.001.
Fig 4.
The comparison of Hmox-1, Gsta1, Gsta3 and Nqo1 mRNA expression between rat ATI-like cells and ATII cells exposed to CSE, and the comparison of these mRNA expression levels between IQOS CSE and Marlboro Red CSE.
Data represent results from three independent experiments. Each experiment was done in duplicate or triplicate wells. *: p < 0.05, **: p < 0.01, ***: p < 0.001.