Fig 1.
Pictures of plants, leaves, flowers, corollas, basal patches, filaments, anthers, pollen, calyxes, and bracts.
G. herbaceum (a, e), G. nelsonii (b, f), the F1 (c, g), the S1 (d, h).
Table 1.
Comparison of morphological traits between G. herbaceum L. × G. nelsonii Fryx. F1 hybrid and its parental genotypes.
Fig 2.
Validation of F1 and S1 by detecting DNA polymorphism, ploidy analysis, and the number of chromosomes.
(a) Microsatellite loci identified by SSR primers including (ⅰ) NAU1157, (ⅱ) NAU1355, (ⅲ) NAU1052, (ⅳ)J ESPR156, (ⅴ) BNL1679, and (ⅵ) NAU3093. The novel bands produced in F1 and S1 are indicated by red and blue arrows (1, G. herbaceum; 2, G. nelsonii; 3, the F1; 4, the S1). (b) G. herbaceum at 200 channels. (c) TM-1 at 400 channels, respectively. (d) F1 and S1 at 200 channels and 400 channels respectively. (e) 26 of G. herbaceum. (f) 26 of G. nelsonii. (g) 26 of the F1. (h) 52 of the S1. The root tip cells (red arrows) and the chromosomes (black arrows) stained with Carbol fuchsin solution under 40× microscope.
Table 2.
SSR polymorphism primer sequences.
Fig 3.
Pictures of cotton bolls and fibers.
(a) G. herbaceum had conical, smooth green and red bolls. (b) G. nelsonii had oval, green cotton bolls. (c) Green pear-shaped boll of S1. (d) G. herbaceum had 3-chambered bolls with gray-white spiral cotton fibers. (e) G. nelsonii had 3-chambered bolls with dark brown upright fibers. (f) The S1 had 3-chambered bolls with brown fibers.
Table 3.
Comparison between S1 and S2 (G. herbaceum × G. nelsonii) generations based on morphological and fertility traits.
Fig 4.
(a) Pink, cylindrical flower with pink corolla, (b) funnel-shaped flower with light pink corolla, (c) funnel-shaped flower with white and pink alternating corolla, (d) white, closed flower, (e) bell-shaped flower with red corolla and a clear rose-colored spot on the petal base, (f) semi-closed flower with pink corolla, (g) bell-shaped flower with pale pink corolla. (h) small flower with white corolla and no spot on the petal base.
Table 4.
Comparison of quantifiable parameters in the S2 descendants of S1 with their parental plants.
Fig 5.
Identification of resistance to Verticillium dahliae Kleb.
(a) The pathogen of the conidia is indicated with the black arrow. (b) Disease-susceptible upland cotton Jimian11, with yellowing, necrosis, and leaf abscission indicated by red arrows. (c) The resistant S1. (d) Resistant G. nelsonii. (e) Resistant G. herbaceum.
Table 5.
Identification of cotton Verticillium wilt resistance in the S2 descendants of S1 with their parental plants.
Fig 6.
The plants, leaves, flowers, corollas, basal patches, filaments, anthers, pollen, calyxes, bracts, and bolls about TM-1, S1 and their offprings.
TM-1(a, e), S1(b, f), F1-1(c, g), F1-2(d, h). (i) normal conical boll of cotton TM-1, (j) green pear-shaped boll of S1, (k) downy green boll of F1-1 that was deformed, indicated by the red arrow, and (l) normal conical boll of F1-2.
Table 6.
Hybridization characteristics of TM-1× (G. herbaceum × G. nelsonii S1).