Table 1.
Brassica genotypes used in the current study, their names, accession numbers, DSI ± SD, vegetation and maturity period, species and genetic background/origin (if known).
Fig 1.
Isolation, purification and identification of A. brassicicola from Brassicaceae.
(a-b) growth of A. brassicicola on PDA media (5 days) (b) growth of A. brassicicola on PDB media (10 days) (c) conidia of A. brassicicola.
Fig 2.
Infection on Brassica plants caused by A. brassicicola.
(a) EC1494 (tolerant B. napus), (b) EC24181 (susceptible B. napus), (c) EC250407 (tolerant B. rapa), (d) EC1333 (susceptible B. rapa) (e) concentric rings surrounded by necrotic lesions (f) matured Brassica leaf covered by black sooty mold of A. brassicicola (g) black spots covered plant siliques and stem (h) plant roots and soil badly affected by black mold of A. brassicicola.
Fig 3.
Molecular and biochemical assays of the A. brassicicola-Brassicaceae pathosystem.
Genotypes (40-day old seedlings) were inoculated with spore suspenion. Samples were taken from 4 hpi to 72 hpi. Processed samples were used for several bioassays comprising of photosynthetic pigments, three redox enzymatic activities and total soluble proteins, with atleast three replication per sample. Bars represent means with standard deviation. Datasets for each parameter were analyzed with 2-way ANOVA (α = 0.05; S2 Table), with posthoc Tukey’s HSD whenever significant (P < 0.05) interaction between genotypes and time (hpi) were recorded. Data points with same letters do not differ significantly.
Fig 4.
Morpho-yield parameters in rapeseed and field mustard.
Bars represent means with standard deviation. Datasets for each parameter were analyzed with 2-way ANOVA (α = 0.05; S3 Table), with posthoc Tukey’s HSD whenever significant (P < 0.05). Data points with same letters do not differ significantly.
Fig 5.
Representation of association among all studied parameters.
YP: yield per plant, TSW: thousand seed weight, SL: silique length, RL: raceme length, PP: Pods per plant, PH: plant height, SP: seeds/pod, CAT: catalase, SOD: superoxide dismutase, POD: peroxidase, Carot: carotenoids, Chla: chlorophyll a, Chlb: Chlorophyll b, Totalchl: total chlorophyll, protein: Total soluble proteins.